Graduation Program in Environmental and Experimental Pathology, Universidade Paulista, São Paulo, Brazil.
IPEN-Institute of Energy and Nuclear Research, São Paulo, Brazil, São Paulo, Brazil.
Homeopathy. 2021 Nov;110(4):244-255. doi: 10.1055/s-0041-1729562. Epub 2021 Sep 2.
Finding solutions to mitigate the impact of pollution on living systems is a matter of great interest. Homeopathic preparations of toxic substances have been described in the literature as attenuation factors for intoxication. Herein, an experimental study using and mercury chloride was developed as a model to identify aspects related to bioresilience.
The aim of the study was to describe the effects of homeopathic (MC) on cysts hatching and on mercury bioavailability.
cysts were exposed to 5.0 µg/mL of mercury chloride during the hatching phase. MC potencies (6cH, 30cH, and 200cH) were prepared in sterile purified water and poured into artificial sea water. Different controls were used (non-challenged cysts and challenged cysts treated with water, succussed water, and 1cH). Four series of nine experiments were performed to evaluate the percentage of cyst hatching. Soluble total mercury (THg) levels and precipitated mercury content were also evaluated. Solvatochromic dyes were used to check for eventual physicochemical markers of MC biological activity.
Significant delay ( < 0.0001) in cyst hatching was observed only after treatment with MC 30cH, compared with controls. This result was associated with an increase of THg concentration in water ( = 0.0018) and of chlorine/oxygen ratio ( < 0.0001) in suspended micraggregates, suggesting changes in mercury bioavailability. A specific interaction of MC 30cH with the solvatochromic dye ET33 ( = 0.0017) was found.
Changes in hatching rate and possible changes in Hg bioavailability are postulated as protective effects of MC 30cH on , by improving its natural bioresilience processes.
寻找减轻污染对生命系统影响的解决方案是一个非常重要的问题。文献中描述了有毒物质的顺势疗法制剂是中毒缓解的因素。在此，我们使用汞和氯化汞作为模型，开发了一项实验研究，以确定与生物弹性相关的方面。
本研究旨在描述顺势疗法汞（MC）对 包囊孵化和汞生物利用度的影响。
在孵化阶段，将 包囊暴露于 5.0 µg/mL 的氯化汞中。MC 效价（6cH、30cH 和 200cH）在无菌纯化水中制备，并倒入人工海水中。使用不同的对照（未受挑战的包囊和用纯水、超声水和 1cH 处理的受挑战的包囊）。进行了四组共九项实验来评估包囊孵化的百分比。还评估了可溶性总汞（THg）水平和沉淀汞含量。使用溶剂化变色染料检查 MC 生物活性的潜在物理化学标志物。
与对照组相比，仅在用 MC 30cH 处理后观察到包囊孵化的明显延迟（ < 0.0001）。这一结果与水中 THg 浓度的增加（ = 0.0018）和悬浮微团聚体中氯/氧比的增加（ < 0.0001）相关，表明汞生物利用度发生了变化。还发现 MC 30cH 与溶剂化变色染料 ET33 之间存在特定的相互作用（ = 0.0017）。
孵化率的变化和 Hg 生物利用度的变化可能被认为是 MC 30cH 对 的保护作用，通过改善其天然生物弹性过程。
