Saxon A, Stevens R H, Quan S G, Golde D W
J Immunol. 1978 Mar;120(3):777-82.
The immunobiologic characteristics of three continuous cell lines established from hairy cell leukemia cells were investigated. All three cell lines continued to produce tartrate-resistant acid phosphatase, the enzymatic marker of hairy cells. Two of these cell lines were B lymphoid in nature. They carried Fc and C receptors, had surface and internal immunoglobulin, and did not form spontaneous sheep red blood cell rosettes. Experiments employing biosynthetic radiolabeling of immunoglobulin demonstrated distinctive immunoglobulin kinetics for each of these two hairy cell lines. One cell line remained quite similar to the original hairy cells from which it was derived whereas the other B lymphoid hairy cell line had undergone a switch in the immunoglobulin isotype produced. The third hairy cell leukemia line was shown to be of thymic derivation. These cells formed spontaneous sheep red blood cell rosettes and did not carry Fc or C receptors. The spontaneous sheep red blood cell rosette-forming cells contained tartrate-resistant acid phosphatase. They did not possess surface on internal immunoglobulin and did not synthesize immunoglobulin in vitro. Hairy cell leukemia cells maintained in permanent cell culture retain their immunobiologic properties and offer the opportunity for indepth study of these unusual cells.
对从毛细胞白血病细胞建立的三个连续细胞系的免疫生物学特性进行了研究。所有这三个细胞系都继续产生抗酒石酸酸性磷酸酶，这是毛细胞的酶标志物。其中两个细胞系本质上是B淋巴细胞系。它们带有Fc和C受体，具有表面和内部免疫球蛋白，并且不形成自发的绵羊红细胞花环。采用免疫球蛋白生物合成放射性标记的实验表明，这两个毛细胞系中的每一个都有独特的免疫球蛋白动力学。一个细胞系与它所源自的原始毛细胞非常相似，而另一个B淋巴细胞性毛细胞系所产生的免疫球蛋白同种型发生了转换。第三个毛细胞白血病系显示来源于胸腺。这些细胞形成自发的绵羊红细胞花环，不带有Fc或C受体。自发形成绵羊红细胞花环的细胞含有抗酒石酸酸性磷酸酶。它们不具有表面或内部免疫球蛋白，并且在体外不合成免疫球蛋白。维持在永久细胞培养中的毛细胞白血病细胞保留了它们的免疫生物学特性，并为深入研究这些异常细胞提供了机会。
