使用 Ni 磁珠实现 SARS-CoV-2 血清转换的超快速、高通量和低成本检测。

Setor Litoral, UFPR Matinhos, PR, Brazil.

Organismic Interactions Department, Interfaculty Institute for Microbiology and Infection Medicine, Cluster of Excellence 'Controlling Microbes to Fight Infections', Tübingen University, Auf der Morgenstelle 28, 72076, Tübingen, Germany.

Anal Biochem. 2021 Oct 15;631:114360. doi: 10.1016/j.ab.2021.114360. Epub 2021 Sep 3.

To monitor the levels of protecting antibodies raised in the population in response to infection and/or to immunization with SARS-CoV-2, we need a technique that allows high throughput and low-cost quantitative analysis of human IgG antibodies reactive against viral antigens. Here we describe an ultra-fast, high throughput and inexpensive assay to detect SARS-CoV-2 seroconversion in humans. The assay is based on Ni magnetic particles coated with His tagged SARS-CoV-2 antigens. A simple and inexpensive 96 well plate magnetic extraction/homogenization process is described which allows the simultaneous analysis of 96 samples and delivers results in 7 min with high accuracy.

为了监测人群中针对 SARS-CoV-2 感染和/或免疫而产生的保护抗体水平，我们需要一种能够高通量、低成本地定量分析针对病毒抗原的人 IgG 抗体的技术。在这里，我们描述了一种用于检测人类 SARS-CoV-2 血清转化的超快速、高通量且廉价的检测方法。该检测方法基于 Ni 磁珠，表面包被 His 标记的 SARS-CoV-2 抗原。本文还描述了一种简单且廉价的 96 孔板磁珠提取/均化处理方法，可同时分析 96 个样本，在 7 分钟内即可获得高精度的结果。