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自动法高估青光眼伴角膜内皮细胞功能不良眼的角膜内皮细胞密度。

Overestimation of corneal endothelial cell density by automated method in glaucomatous eyes with impaired corneal endothelial cells.

机构信息

Sensho-kai Eye institute, Minamiyama 50-1, Iseda, Uji, Kyoto, 611-0043, Japan.

Minami Eye Clinic, Yokaichi Midorimachi 1-7, Higashi-omi, Shiga, 527-0023, Japan.

出版信息

Int Ophthalmol. 2022 Jan;42(1):133-145. doi: 10.1007/s10792-021-02008-4. Epub 2021 Sep 5.

DOI:10.1007/s10792-021-02008-4
PMID:34482487
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8803627/
Abstract

PURPOSE

To determine between-method differences in corneal endothelial cell parameters using center and automated methods of non-contact specular microscopy (CellCheck software of Konan, Inc.) in glaucomatous eyes.

METHODS

We analyzed the central corneal endothelial cell density (ECD) of 245 glaucomatous eyes using center (ECD-Ce) and automated methods (ECD-Au). Based on the ECD-Ce, we allocated subjects to Groups 1 to 10 (at 250 cells/mm intervals) and evaluated the ECD, coefficient of variation in cell area (CV), and percentage of hexagonal cells (HEX).

RESULTS

There was a close correlation (r = 0.91) between the ECD values measured using both methods. However, ECD-Au were significantly higher than those measured by the center method when ECD-Ce was less than 2500 (in Groups 1 to 8; P < 0.001 to P = 0.006). The regression equation of (ECD-Au-ECD-Ce) = 1028-0.397*ECD-Ce shows greater deviation in eyes with lower ECD, and this difference became 0 when ECD -Ce was 2593 cells/mm. None of the 44 subjects with an ECD-Ce of < 1000 cells/mm recorded an ECD-Au < 1000 cells/mm. Compared with the center method, the automated method had higher and lower median CV and HEX values, respectively (P < 0.001). The between-method differences in both CV and HEX were negatively correlated with ECD-Ce (r = -0.49, P < 0.001 and r = -0.25, P < 0.001, respectively).

CONCLUSION

The automated method of the CellCheck software overestimates ECD in eyes with lower ECD values and may overlook risk of corneal decompensation.

摘要

目的

使用非接触式共焦显微镜（Konan 公司的 CellCheck 软件）的中心和自动化方法，确定青光眼眼中角膜内皮细胞参数的方法间差异。

方法

我们使用中心（ECD-Ce）和自动化方法（ECD-Au）分析了 245 只青光眼眼的中央角膜内皮细胞密度（ECD）。根据 ECD-Ce，我们将受试者分配到 1 到 10 组（以 250 个细胞/mm 为间隔），并评估 ECD、细胞面积变异系数（CV）和六边形细胞百分比（HEX）。

结果

两种方法测量的 ECD 值密切相关（r=0.91）。然而，当 ECD-Ce 小于 2500 时（在 1 到 8 组中；P<0.001 至 P=0.006），ECD-Au 明显高于中心法测量值。（ECD-Au-ECD-Ce）=1028-0.397*ECD-Ce 的回归方程表明，在 ECD 较低的眼睛中，偏差较大，当 ECD-Ce 为 2593 个细胞/mm 时，差异为 0。在 ECD-Ce<1000 个细胞/mm 的 44 名受试者中，无一例记录 ECD-Au<1000 个细胞/mm。与中心法相比，自动化方法的 CV 和 HEX 的中位数分别较高和较低（P<0.001）。CV 和 HEX 的方法间差异与 ECD-Ce 呈负相关（r=-0.49，P<0.001 和 r=-0.25，P<0.001）。

结论

CellCheck 软件的自动化方法在 ECD 值较低的眼中高估 ECD，可能会忽略角膜失代偿的风险。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/771b/8803627/a89f92318e4a/10792_2021_2008_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/771b/8803627/017b704dcd36/10792_2021_2008_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/771b/8803627/59bb920c0921/10792_2021_2008_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/771b/8803627/dba5fbe998f2/10792_2021_2008_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/771b/8803627/e18b96869be6/10792_2021_2008_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/771b/8803627/06442b751c3c/10792_2021_2008_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/771b/8803627/c867f751b64a/10792_2021_2008_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/771b/8803627/7c19c852b05d/10792_2021_2008_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/771b/8803627/8f47a858b4ec/10792_2021_2008_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/771b/8803627/a89f92318e4a/10792_2021_2008_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/771b/8803627/017b704dcd36/10792_2021_2008_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/771b/8803627/59bb920c0921/10792_2021_2008_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/771b/8803627/dba5fbe998f2/10792_2021_2008_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/771b/8803627/e18b96869be6/10792_2021_2008_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/771b/8803627/06442b751c3c/10792_2021_2008_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/771b/8803627/c867f751b64a/10792_2021_2008_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/771b/8803627/7c19c852b05d/10792_2021_2008_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/771b/8803627/8f47a858b4ec/10792_2021_2008_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/771b/8803627/a89f92318e4a/10792_2021_2008_Fig9_HTML.jpg

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