Molecular Medicine and Tumor Research Center, 12550Chongqing Medical University, Chongqing, China.
Southwest Hospital, 12525First Affiliated Hospital of Army Military Medical University, Chongqing, China.
Technol Cancer Res Treat. 2021 Jan-Dec;20:15330338211039678. doi: 10.1177/15330338211039678.
The incidence of colorectal cancer (CRC), as well as subsequent patient mortality, has increased in the last decade; an unhealthy diet is considered to be the leading cause. Previous studies have shown the potential of the bromodomain containing 1 () gene as a therapeutic target for CRC based on its specificity; however, the genetic mode of action and expression in CRC cells are yet to be investigated. In this study, target genes were screened from single-cell transcriptome sequencing data, and the collected clinical specimens were subjected to immunohistochemistry (IHC) to identify the protein expression of target genes; the results were verified in the GSE17536 array set. Receiver operating characteristic curves (ROC) and overall survival (OS) were used to test target genes as biomarkers and independent predictive markers for CRC. Based on these results, was screened as a target gene, and IHC results showed that BRD1 protein expression in CRC was higher than that in normal tissues and was significantly upregulated in poorly differentiated (PD) CRC. ROC analysis showed that the area under the curve in the collected clinical specimens and GSE17536 were 0.6062 and 0.6094, respectively. OS analysis showed that higher BRD1 protein expression was associated with a significantly shorter survival time. In conclusion, BRD1 expression was positively correlated with PD CRC and negatively correlated with OS, indicating that could predict the differentiation state of CRC and may be a novel predictive biomarker.
结直肠癌（CRC）的发病率以及随后的患者死亡率在过去十年中有所增加；不健康的饮食被认为是主要原因。先前的研究表明，溴结构域蛋白 1（）基因作为 CRC 的治疗靶点具有特异性；然而，其在 CRC 细胞中的遗传作用模式和表达尚未得到研究。在本研究中，从单细胞转录组测序数据中筛选靶基因，并对收集的临床标本进行免疫组织化学（IHC）鉴定靶基因的蛋白表达；在 GSE17536 数组集中验证结果。接收者操作特征曲线（ROC）和总生存期（OS）用于测试靶基因作为 CRC 的生物标志物和独立预测标志物。基于这些结果，筛选出作为靶基因，IHC 结果表明，CRC 中 BRD1 蛋白的表达高于正常组织，在低分化（PD）CRC 中显著上调。ROC 分析显示，在收集的临床标本和 GSE17536 中的曲线下面积分别为 0.6062 和 0.6094。OS 分析表明，BRD1 蛋白表达越高，生存时间越短。总之，BRD1 表达与 PD CRC 呈正相关，与 OS 呈负相关，表明可能预测 CRC 的分化状态，可能是一种新的预测生物标志物。
