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含羟基磷灰石的胶原基支架对兔膝关节软骨缺损修复的影响。

Effect of collagen-based scaffolds with hydroxyapatite on the repair of cartilage defects in the rabbit knee joint.

作者信息

He Xiaoliang, Han Qiuping, Zhang Yuxin, Zhang Huan, Liu Jun, Zhou Xiaohui

机构信息

College of Food Science and Biology, Hebei University of Science and Technology, NO. 26 Yuxiang Street, Shijiazhang, Hebei, 050018, China.

College of Food and Biotechnology, Qiqihar University, Qiqihaer, Heilongjiang, China.

出版信息

J Orthop Surg Res. 2024 Dec 3;19(1):818. doi: 10.1186/s13018-024-05323-5.

DOI:10.1186/s13018-024-05323-5
PMID:39623498
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11613844/
Abstract

BACKGROUND

The repair of articular cartilage defects is always a significant clinical challenge in joint treatment. Therefore, the aim of this study was to investigate that the ColII-HA-CS-HAP scaffolds with BMSCs could repair cartilage defects of knee.

METHODS

Bone marrow mesenchymal stem cells (BMSCs) were extracted from rabbits, identified using immunofluorescence staining, and successfully induced into chondrocytes. Type II collagen (ColII) was isolated from bovine cartilage and constructed into scaffolds with hyaluronic acid, chondroitin sulfate, and hydroxyapatite. Then BMSCs were seeded on the ColII-HA-CS-HAP scaffold to detect biocompatibility.

RESULTS

The results of DAPI fluorescence staining showed that the number of BMSCs on the ColII-HA-CS-HAP scaffolds increased rapidly after culturing for 12 d. The rabbit knee cartilage defect model with a diameter of approximately 3 mm and a thickness of approximately 4 mm was selected to evaluate the regenerative potential of the scaffolds using histological and immunohistochemical analyses. At 6 months, the regenerated cartilage in the ColII-HA-CS-HAP scaffolds with BMSCs was more similar to that of native cartilage than the ColII-HA-CS-HAP scaffold group.

CONCLUSIONS

Our study proved that the ColII-HA-CS-HAP scaffolds with differentiated BMSCs can produce an excellent healing response and repair cartilage defects successfully in a rabbit model.

摘要

背景

关节软骨缺损的修复一直是关节治疗中的一项重大临床挑战。因此，本研究的目的是探究负载骨髓间充质干细胞（BMSCs）的II型胶原蛋白-透明质酸-硫酸软骨素-羟基磷灰石（ColII-HA-CS-HAP）支架能否修复兔膝关节软骨缺损。

方法

从兔体内提取骨髓间充质干细胞，采用免疫荧光染色进行鉴定，并成功诱导分化为软骨细胞。从牛软骨中分离出II型胶原蛋白，与透明质酸、硫酸软骨素和羟基磷灰石构建成支架。然后将骨髓间充质干细胞接种到ColII-HA-CS-HAP支架上检测其生物相容性。

结果

4',6-二脒基-2-苯基吲哚（DAPI）荧光染色结果显示，培养12天后，ColII-HA-CS-HAP支架上的骨髓间充质干细胞数量迅速增加。选取直径约3 mm、厚度约4 mm的兔膝关节软骨缺损模型，采用组织学和免疫组织化学分析评估支架的再生潜力。6个月时，与ColII-HA-CS-HAP支架组相比，负载骨髓间充质干细胞的ColII-HA-CS-HAP支架再生的软骨与天然软骨更相似。

结论

我们的研究证明，负载分化骨髓间充质干细胞的ColII-HA-CS-HAP支架能够产生良好的愈合反应，并在兔模型中成功修复软骨缺损。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e22d/11613844/91a17105e4e1/13018_2024_5323_Fig7_HTML.jpg
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