Emu Quzhe, Guan Hao, Zhu Jiangjiang, Zhang Lin, Fan Jinsheng, Ji Yang, Lin Yaqiu, Li Chunmei, Dan Xiaobo, Aguo Yueda, Wei Xiaolan, Zhang Min, Zhang Bin, Yang Chao, Li Bo, Xiong Chaorui
Animal Breeding and Genetics Key Laboratory of Sichuan Province, Sichuan Animal Science Academy, Chengdu, China.
Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization Key Laboratory of Sichuan Province, Southwest Minzu University, Chengdu, China.
Front Microbiol. 2021 Aug 18;12:666837. doi: 10.3389/fmicb.2021.666837. eCollection 2021.
This study aimed to investigate the effects of different feeding modes on the growth performance, gut microbiota, and immunity of Black Fattening Goat (). A total of 30 goats were grouped in three groups by their feeding modes (pasture grazing group, PG; barn feeding group, BF; barn feeding + probiotics, BF + P; = 10) and the study was performed for 114 days. After a 2-week adaptation period, the first growth performance test was conducted, and the blood and fecal samplings (day 0) were collected on January 17, 2020, while the second and third test and samplings were conducted on days 53 and 100 of feeding. The species-composition of fecal microbiota was analyzed by 16S ribosomal RNA gene-sequencing using PacBio single molecule real time (SMRT) sequencing technology. Both the BF and BF + P groups had the highest ( < 0.05) body's weight and length, and chest circumference at days 53 and 100, especially at day 100, the body's weight of both the BF groups were more than 18 kg. The levels of immunoglobulin A (IgA) and immunoglobulin G (IgG) were found to be significantly higher ( < 0.05) in the PG and BF + P groups at day 100. The PG group exhibited the highest number of operational taxonomic unit (OTUs) and alpha diversity. Firmicutes, Bacteroidetes, and Verrucomicrobia were the predominant phyla in all the fecal samples. The relative abundance of and were found to be significantly higher ( < 0.05) in PG group and BF + P group at day 100, respectively, which might partially explain the significantly higher ( < 0.05) levels of IgA and IgG in these two groups. These findings suggested that BF supplemented with 5 g probiotics ( and mannan oligosaccharides) per day has the potential to enhance the growth and immunity of Black Fattening Goats.
本研究旨在探讨不同饲养模式对黑山羊育肥期生长性能、肠道微生物群和免疫力的影响。将30只山羊按饲养模式分为三组（放牧组，PG；舍饲组，BF；舍饲+益生菌组，BF+P；每组n = 10），试验期为114天。经过2周的适应期后，进行首次生长性能测试，并于2020年1月17日采集血液和粪便样本（第0天），第二次和第三次测试及样本采集分别在饲养的第53天和第100天进行。采用PacBio单分子实时（SMRT）测序技术，通过16S核糖体RNA基因测序分析粪便微生物群的物种组成。BF组和BF+P组在第53天和第100天的体重、体长和胸围均最高（P < 0.05），尤其是在第100天，两个BF组的体重均超过18 kg。在第100天，PG组和BF+P组的免疫球蛋白A（IgA）和免疫球蛋白G（IgG）水平显著更高（P < 0.05）。PG组的可操作分类单元（OTU）数量和α多样性最高。厚壁菌门、拟杆菌门和疣微菌门是所有粪便样本中的主要门类。在第100天，PG组和BF+P组的[具体菌属]相对丰度分别显著更高（P < 0.05），这可能部分解释了这两组中IgA和IgG水平显著更高（P < 0.05）的原因。这些结果表明，每天补充5 g益生菌（[具体菌属]和甘露寡糖）的舍饲模式有潜力提高黑山羊育肥期的生长性能和免疫力。
