Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China; Cotton Research Center, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China.
J Proteomics. 2019 Jun 15;201:73-83. doi: 10.1016/j.jprot.2019.04.017. Epub 2019 Apr 19.
Sesame is one of the most important oilseed crops and has high nutritional value. The yield and quality of sesame are severely affected by high salinity in coastal and semi-arid/arid regions. In this study, the phenotypic, physiological, and proteomic changes induced by salt treatment were analyzed in salt-tolerant (G441) and salt-sensitive (G358) seedlings. Phenotypic and physiological results indicated that G441 had an enhanced capacity to withstand salinity stress compared to G358. Proteomic analysis revealed a strong induction of salt-responsive protein species in sesame, mainly related to catalytic, hydrolase, oxidoreductase, and binding activities. Pathway enrichment analysis showed that more salt-responsive proteins in G441 were involved in tyrosine metabolism, carbon fixation in photosynthetic organisms, carbon metabolism, alpha-linolenic acid metabolism, biosynthesis of amino acids, photosynthesis, and glutathione metabolism. Furthermore, G441 displayed unique differentially accumulated proteins in seedlings functioning as heat shock proteins, abscisic acid receptor PYL2-like, calcium-dependent protein kinases, serine/threonine-protein phosphatases, nucleoredoxin, and antioxidant enzymes. Quantitative real-time PCR analysis revealed that some of the proteins were also regulated by salinity stress at the transcript level. Our findings provide important information on salinity responses in plants and may constitute useful resources for enhancing salinity tolerance in sesame. SIGNIFICANCE: Our study identified potential biological pathways and salt-responsive protein species related to transducing stress signals and scavenging reactive oxygen species under salt stress. These findings will provide possible participants/pathways/proteins that contribute to salt tolerance and may serve as the basis for improving salinity tolerance in sesame and other plants.
芝麻是最重要的油料作物之一，具有很高的营养价值。在沿海和半干旱/干旱地区，高盐度严重影响芝麻的产量和品质。本研究分析了耐盐（G441）和盐敏感（G358）幼苗在盐处理下的表型、生理和蛋白质组变化。表型和生理结果表明，G441 比 G358 具有更强的耐盐能力。蛋白质组分析显示，芝麻中强烈诱导了盐响应蛋白，主要与催化、水解酶、氧化还原酶和结合活性有关。途径富集分析表明，G441 中更多的盐响应蛋白参与了酪氨酸代谢、光合生物中的碳固定、碳代谢、α-亚麻酸代谢、氨基酸生物合成、光合作用和谷胱甘肽代谢。此外，G441 在幼苗中表现出独特的差异积累蛋白，这些蛋白作为热休克蛋白、ABA 受体 PYL2 样、钙依赖性蛋白激酶、丝氨酸/苏氨酸蛋白磷酸酶、核还原酶和抗氧化酶发挥作用。定量实时 PCR 分析显示，一些蛋白质在转录水平也受到盐胁迫的调节。我们的研究结果提供了植物对盐胁迫反应的重要信息，可能为提高芝麻的耐盐性提供有用的资源。意义：本研究鉴定了与在盐胁迫下传递胁迫信号和清除活性氧有关的潜在生物学途径和盐响应蛋白种类。这些发现将为耐盐性提供可能的参与者/途径/蛋白，并可能为提高芝麻和其他植物的耐盐性提供基础。
