Department of Molecular Biotechnology, Institute of Agricultural Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
Department of Plant Biology, Faculty of Biosciences, Kharazmi University, Tehran, Iran.
GM Crops Food. 2021 Jan 2;12(1):419-433. doi: 10.1080/21645698.2021.1954467. Epub 2021 Sep 8.
Rhizomania is an economically important disease of sugar beet, which is caused by (BNYVV). As previously shown, RNA silencing mechanism effectively inhibit the viral propagation in transgenic sugar beet plants. To investigate possible proteomic changes induced by gene insertion and/or RNA silencing mechanism, the root protein profiles of wild type sugar beet genotype 9597, as a control, and transgenic events named 6018-T3:S6-44 (S6) and 219-T3:S3-13.2 (S3) were compared by two-dimensional gel electrophoresis. The accumulation levels of 25 and 24 proteins were differentially regulated in S3 and S6 plants, respectively. The accumulation of 15 spots were increased or decreased more than 2-fold. Additionally, 10 spots repressed or induced in both, while seven spots showed variable results in two events. All the differentially expressed spots were analyzed by MALDI-TOF-TOF mass spectrometry. The functional analysis of differentially accumulated proteins showed that most of them are related to the metabolism and defense/stress response. None of these recognized proteins were allergens or toxic proteins except for a spot identified as phenylcoumaran benzylic ether reductase, Pyrc5, which was decreased in the genetically modified S6 plant. These data are in favor of substantial equivalence of the transgenic plants in comparison to their related wild type cultivar since the proteomic profile of sugar beet root was not remarkably affected by gene transfer and activation RNA silencing mechanism.
黄脉病毒病是甜菜的一种重要经济病害，由（BNYVV）引起。如前所述，RNA 沉默机制可有效抑制转基因甜菜植株中的病毒繁殖。为了研究基因插入和/或 RNA 沉默机制可能引起的蛋白质组变化，通过二维凝胶电泳比较了野生型甜菜基因型 9597（对照）和转基因事件 6018-T3：S6-44（S6）和 219-T3：S3-13.2（S3）的根蛋白图谱。S3 和 S6 植物中分别有 25 和 24 种蛋白质的积累水平受到差异调控。15 个斑点的积累增加或减少了 2 倍以上。此外，10 个斑点在两个事件中均被抑制或诱导，而 7 个斑点在两个事件中的结果不同。所有差异表达的斑点均通过 MALDI-TOF-TOF 质谱进行分析。差异积累蛋白的功能分析表明，它们大多数与代谢和防御/应激反应有关。除了一个被鉴定为苯并呋喃苄醚还原酶（Pyrc5）的斑点在转基因 S6 植物中减少外，这些识别出的蛋白质均不是过敏原或毒性蛋白。这些数据表明，与相关的野生型品种相比，转基因植物具有实质性等同性，因为基因转移和激活 RNA 沉默机制并未显著影响甜菜根的蛋白质组图谱。
