R&D Coordination Division, Rural Development Administration, Jeollabuk-do, Republic of Korea.
National Institute of Agricultural Sciences, Rural Development Administration, Jeollabuk-do, Republic of Korea.
GM Crops Food. 2021 Jan 2;12(1):303-314. doi: 10.1080/21645698.2021.1893624.
The safety of transgenic Bt rice containing bacteria-derived gene from (Bt) was assessed by conducting field trials at two locations for two consecutive years in South Korea, using the near-isogenic line comparator rice cultivar ('Ilmi', non-Bt rice) and four commercial cultivars as references. Compositional analyses included measurement of proximates, minerals, amino acids, fatty acids, vitamins, and antinutrients. Significant differences between Bt rice and non-Bt rice were detected; however, all differences were within the reference range. The statistical analyses, including analysis of % variability, analysis of similarities (ANOISM), similarity percentage (SIMPER) analysis, and permutational multivariate analysis of variance (PERMANOVA) were performed to study factors contributing to compositional variability. The multivariate analyses revealed that environmental factors more influenced rice components' variability than by genetic factors. This approach was shown to be a powerful method to provide meaningful evaluations between Bt rice and its comparators. In this study, Bt rice was proved to be compositionally equivalent to conventional rice varieties through multiple statistical methods.
韩国在两个地点连续两年进行田间试验，评估了含有细菌衍生基因的转 Bt 水稻的安全性，使用了近等基因系对照水稻品种（“Ilmi”，非 Bt 水稻）和四个商业品种作为参考。成分分析包括对粗蛋白、矿物质、氨基酸、脂肪酸、维生素和抗营养因子的测量。在转 Bt 水稻和非 Bt 水稻之间检测到了显著差异；然而，所有差异都在参考范围内。进行了统计分析，包括%变异性分析、相似性分析（ANOISM）、相似性百分比（SIMPER）分析和可置换多元方差分析（PERMANOVA），以研究导致成分变异性的因素。多变量分析表明，环境因素比遗传因素更能影响水稻成分的变异性。这种方法被证明是一种有力的方法，可以在 Bt 水稻与其对照之间进行有意义的评估。在这项研究中，通过多种统计方法证明 Bt 水稻在成分上与常规水稻品种等效。
