从日本临床样本中分离出的组的三个新种。

Three novel species of the group isolated from clinical samples in Japan.

机构信息

Department of Microbiology, Juntendo University School of Medicine, Tokyo, Japan.

Department of Microbiome Research, Juntendo University School of Medicine, Tokyo, Japan.

出版信息

Int J Syst Evol Microbiol. 2021 Sep;71(9). doi: 10.1099/ijsem.0.004993.

DOI:10.1099/ijsem.0.004993

PMID:34494947

Abstract

Three Gram-positive bacterial strains, BML-BC004, BML-BC017 and BML-BC059, isolated from blood samples from three inpatients in Japan, were identified as members of using matrix-assisted laser desorption ionization time-of-flight MS. The 16S rRNA gene sequences of these three strains were more than 97.1 % similar to 18 type strains belonging to the group. Whole-genome comparisons, using average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH), confirmed that the three strains represented three individual distinct species belonging to the group. A phylogenetic tree showed that BML-BC004, BML-BC017 and BML-BC059 were located close to , and , respectively. Based on these phylogenetic and phenotypic data, including values below the threshold for ANI and dDDH, the three strains should be classified as representing three different novel species of the group: sp. nov., with type strain BML-BC004 (=DSM 111102=JCM 34122), sp. nov., with type strain BML-BC017 (=DSM 111100=JCM 34124) and sp. nov., with type strain BML-BC059 (=DSM 111101=JCM 34125).

摘要

从日本的三名住院患者的血液样本中分离出的三株革兰氏阳性细菌菌株 BML-BC004、BML-BC017 和 BML-BC059，通过基质辅助激光解吸电离飞行时间质谱法被鉴定为属于。这三株菌的 16S rRNA 基因序列与属于组的 18 个参考株的相似度超过 97.1%。全基因组比较，使用平均核苷酸同一性 (ANI) 和数字 DNA-DNA 杂交 (dDDH)，证实这三株菌代表属于组的三个不同的独特种。系统发育树表明 BML-BC004、BML-BC017 和 BML-BC059 分别与、和密切相关。基于这些系统发育和表型数据，包括低于 ANI 和 dDDH 阈值的值，这三株菌应被归类为代表组的三个不同的新型种： sp. nov.，其模式菌株为 BML-BC004（=DSM 111102=JCM 34122）、 sp. nov.，其模式菌株为 BML-BC017（=DSM 111100=JCM 34124）和 sp. nov.，其模式菌株为 BML-BC059（=DSM 111101=JCM 34125）。