Analytical Methods for Detection of Gasotransmitter Hydrogen Sulfide Released from Live Cells.

Hydrogen sulfide (HS) plays an important role in mammals as a signaling molecule. Recently, abnormal HS concentration has been associated with several pathophysiological states, such as diabetes mellitus, hypertension, Alzheimer's disease, and Parkinson's disease. As regulating HS concentration can be a very prominent way of developing new drugs, many researchers have paid great attention to HS research. To understand the role of HS in pathophysiology and develop HS-based therapies, it is necessary to measure the exact concentration of HS within biological systems. But, HS is volatile and can be easily oxidized. Besides, the active sites for several biological effects of HS are inside the cell. Therefore, there is a need for the development of new methods for the accurate and reliable detection of HS within live cells. This review provides a summary of recent developments in HS detection methods for live cell analysis.