Department of Medical Zoology, College of Medicine, Kyung Hee University, Seoul 02447, Republic of Korea.
Department of Biomedical Engineering, College of Medicine, Kyung Hee University, Seoul 02447, Republic of Korea.
Biomed Res Int. 2021 Aug 28;2021:5473965. doi: 10.1155/2021/5473965. eCollection 2021.
Hydrogen sulfide (HS) plays an important role in mammals as a signaling molecule. Recently, abnormal HS concentration has been associated with several pathophysiological states, such as diabetes mellitus, hypertension, Alzheimer's disease, and Parkinson's disease. As regulating HS concentration can be a very prominent way of developing new drugs, many researchers have paid great attention to HS research. To understand the role of HS in pathophysiology and develop HS-based therapies, it is necessary to measure the exact concentration of HS within biological systems. But, HS is volatile and can be easily oxidized. Besides, the active sites for several biological effects of HS are inside the cell. Therefore, there is a need for the development of new methods for the accurate and reliable detection of HS within live cells. This review provides a summary of recent developments in HS detection methods for live cell analysis.
硫化氢 (HS) 在哺乳动物中作为一种信号分子发挥着重要作用。最近，异常的 HS 浓度与多种病理生理状态有关，如糖尿病、高血压、阿尔茨海默病和帕金森病。由于调节 HS 浓度可能是开发新药的一种非常突出的方式，许多研究人员对 HS 研究给予了极大的关注。为了了解 HS 在病理生理学中的作用并开发基于 HS 的治疗方法，有必要测量生物系统中 HS 的准确浓度。但是，HS 是挥发性的，很容易被氧化。此外，HS 的几种生物学效应的活性部位在细胞内。因此，需要开发新的方法来准确可靠地检测活细胞内的 HS。本文综述了用于活细胞分析的 HS 检测方法的最新进展。
