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化学成分分析、体外生物活性及新鲜与干燥缬草花精油的比较

Chemical Profile, In Vitro Biological Activity and Comparison of Essential Oils from Fresh and Dried Flowers of L.

机构信息

Department of Bioscience and Territory, University of Molise, C.da Fonte Lappone snc, 86090 Pesche, IS, Italy.

出版信息

Molecules. 2021 Sep 1;26(17):5317. doi: 10.3390/molecules26175317.

Abstract

The chemical composition of essential oils (EOs) from dried and fresh flowers of L. (lavender), named and respectively, grown in central Italy was analyzed and compared by GC and GC-MS. For both samples, 61 compounds were identified, corresponding to 97.9% and 98.1% of the total essential oils. Explorative data analysis, performed to compare the statistical composition of the samples, resulted in a high level of global similarity (around 93%). The compositions of both samples were characterized by 10 major compounds, with a predominance of Linalool (35.3-36.0%), Borneol (15.6-19.4%) and 1,8-Cineole (11.0-9.0%). The in vitro antibacterial activity assay by disk diffusion tests against PY79 and DH5α showed inhibition of growth in both indicator strains. In addition, plate counts revealed a bactericidal effect on which was particularly noticeable when using oil from the fresh lavender flowers at the highest concentrations. An in vitro antifungal assay showed that the EOs inhibited the growth of , a phytopathogenic fungus that causes post-harvest diseases in many fruits and vegetables. The antioxidant activity was also assessed using the ABTS free radical scavenging assay, which showed a different antioxidant activity in both EOs. In addition, the potential application of EOs as a green method to control biodeterioration phenomena on an artistic wood painting (XIX century) was evaluated.

摘要

对产自意大利中部的干花和鲜花薰衣草(Lavandula angustifolia Mill.)的精油(EOs)的化学成分进行了分析和比较,分别命名为 和 。对于这两个样本,共鉴定出 61 种化合物,分别占精油总量的 97.9%和 98.1%。为了比较样品的统计组成,进行了探索性数据分析,结果表明两者具有高度的整体相似性(约 93%)。这两个样品的组成都以 10 种主要化合物为特征,其中芳樟醇(35.3-36.0%)、龙脑(15.6-19.4%)和 1,8-桉叶素(11.0-9.0%)占主导地位。通过圆盘扩散试验对 PY79 和 DH5α 两种指示菌进行体外抗菌活性测定,结果表明两种精油均能抑制两种指示菌的生长。此外,平板计数法显示,对 具有杀菌作用,特别是使用新鲜薰衣草精油时,在最高浓度下效果更为显著。体外抗真菌试验表明,精油抑制了植物病原菌 的生长, 会导致许多水果和蔬菜的采后病害。还使用 ABTS 自由基清除试验评估了抗氧化活性,结果表明两种精油具有不同的抗氧化活性。此外,还评估了精油作为一种绿色方法控制 XIX 世纪艺术木画生物降解现象的潜在应用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e6c/8434377/73d92cb84656/molecules-26-05317-g001.jpg

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