Niebling C R, Johnson K, Gerhold H D
Institute of Forest Genetics, Pacific Southwest Forest & Range Experiment Station, Berkeley, California 94701.
Biochem Genet. 1987 Dec;25(11-12):803-14. doi: 10.1007/BF00502600.
Eighty megagametophytes from each of 24 Scots pines (Pinus sylvestris L.) were subjected to horizontal starch gel electrophoresis. The trees were from crosses among widely separated provenances, and each was polymorphic for 8 to 14 loci. Evidence for linkage among 275 two-locus combinations was tested using chi-square analysis. Data from different trees were pooled to calculate map distances for the species. Nineteen of the twenty-nine loci tested were linked in one of six groups; the groups varied in size from two to seven loci. Similarities in linkage relationships among Scots pine, other pines, and other species within the Pinaceae support karyological research that suggests extensive conservation of the conifer genome.
对24株欧洲赤松（Pinus sylvestris L.）中每株的80个雌配子体进行水平淀粉凝胶电泳。这些树来自广泛分布的种源之间的杂交，每株在8至14个基因座上具有多态性。使用卡方分析检验了275个双基因座组合之间的连锁证据。汇总来自不同树木的数据以计算该物种的图谱距离。所测试的29个基因座中有19个在六个组中的一组中连锁；这些组的大小从两个到七个基因座不等。欧洲赤松、其他松树以及松科内其他物种之间连锁关系的相似性支持了染色体学研究，该研究表明针叶树基因组具有广泛的保守性。
