Rinaman L, Miselis R R
School of Veterinary Medicine, University of Pennsylvania, Philadelphia 19104.
J Auton Nerv Syst. 1987 Dec;21(2-3):109-25. doi: 10.1016/0165-1838(87)90014-2.
The present study was initiated to address the current controversy concerning the parasympathetic innervation of the pancreas, using a more sensitive tracer. The location of retrogradely labeled neurons within the dorsal motor nucleus of the vagus (DMV) was examined 48 h following injections of cholera toxin-horseradish peroxidase (CT-HRP) into designated areas of the rat pancreas. The brainstem and spinal cord were searched for any additional labeled neurons located outside of the DMV. Separate groups of animals were used for control injections into the adipose tissue of the greater omentum, the spleen, abdominal musculature, and the diaphragm. In addition, CT-HRP was dripped over the surfaces of the abdominal viscera in another group of animals. These control cases were designed to indicate whether diffusion of the neural tracer away from injection sites had occurred and had resulted in labeling of neurons which did not innervate the injected areas. Following injection of CT-HRP into the right lobe of the pancreas, labeled neurons were found primarily within the medial region of the left DMV. Injection of CT-HRP into the left lobe of the pancreas resulted in retrogradely labeled neurons predominantly within the medial region of the right DMV. Following injections into the entire pancreas, neural labeling was seen bilaterally within the DMV and was concentrated within the medial regions, with a slightly higher degree of labeling within the right DMV. No labeled neurons were seen within the nucleus ambiguus or other areas of the brainstem or spinal cord following pancreatic injections. Furthermore, no afferent labeling within the nucleus of the solitary tract (NTS) was observed, although a very small number of neurons within the nodose ganglia were labeled. The dendrites of backfilled DMV neurons could be seen extending across the midline to the contralateral DMV as well as dorsally into certain subnuclei of the NTS, and to the borders of the area postrema and the fourth ventricle. These results indicate that both the motor and sensory innervation of the rat pancreas are more restricted than has been previously suggested.
本研究旨在使用更灵敏的示踪剂，解决当前关于胰腺副交感神经支配的争议。在将霍乱毒素 - 辣根过氧化物酶（CT - HRP）注射到大鼠胰腺的指定区域48小时后，检查迷走神经背核（DMV）内逆行标记神经元的位置。在脑干和脊髓中寻找位于DMV之外的任何其他标记神经元。使用单独的动物组进行向大网膜脂肪组织、脾脏、腹部肌肉组织和膈肌的对照注射。此外，在另一组动物中，将CT - HRP滴在腹部内脏表面。这些对照病例旨在表明神经示踪剂是否已从注射部位扩散，并导致未支配注射区域的神经元被标记。将CT - HRP注射到胰腺右叶后，主要在左侧DMV的内侧区域发现标记神经元。将CT - HRP注射到胰腺左叶后，逆行标记的神经元主要位于右侧DMV的内侧区域。向整个胰腺注射后，在DMV双侧可见神经标记，并集中在内侧区域，右侧DMV内的标记程度略高。胰腺注射后，在疑核或脑干或脊髓的其他区域未发现标记神经元。此外，尽管在结状神经节内有极少数神经元被标记，但在孤束核（NTS）内未观察到传入标记。回充的DMV神经元的树突可以看到从中线延伸到对侧DMV，以及背侧延伸到NTS的某些亚核，以及最后区和第四脑室的边界。这些结果表明，大鼠胰腺的运动和感觉神经支配比以前认为的更受限制。
