Reproductive Medicine Center, Shenyang Women's and Children's Hospital, No. 87 Danan Street, Shenhe District, Shenyang, 110000, Liaoning, China.
Department of Radiology, Shenyang Women's and Children's Hospital, Shenyang, Liaoning, China.
J Ovarian Res. 2021 Sep 10;14(1):118. doi: 10.1186/s13048-021-00859-0.
Circular RNAs (circRNAs) are gradually reported to be implicated in the development of malignant tumors, including ovarian cancer (OC). This paper intended to explore the function and action mechanism of hsa_circ_0004712 in OC.
In our results, hsa_circ_0004712 was aberrantly overexpressed in OC tissues and cells. Downregulation of hsa_circ_0004712 impaired OC cell proliferation, colony formation, invasion and migration, and accelerated apoptosis. Hsa_circ_0004712 directly targeted miR-331-3p whose inhibitors reversed the effects of hsa_circ_0004712 downregulation. FZD4 was targeted by miR-331-3p, and hsa_circ_0004712 could positively regulated FZD4 expression by targeting miR-331-3p. The anti-tumor effects of miR-331-3p restoration were reversed by FZD4 overexpression. Downregulation of hsa_circ_0004712 also impaired tumor development in vivo by regulating miR-331-3p and FZD4.
In conclusion, hsa_circ_0004712 deficiency repressed OC development by mediating the miR-331-3p/FZD4 pathway, predicting that hsa_circ_0004712 was a promising biomarker for OC diagnosis and therapy.
环状 RNA（circRNAs）逐渐被报道与恶性肿瘤的发展有关，包括卵巢癌（OC）。本文旨在探讨 hsa_circ_0004712 在 OC 中的功能和作用机制。
在我们的研究结果中，hsa_circ_0004712 在 OC 组织和细胞中异常高表达。下调 hsa_circ_0004712 抑制 OC 细胞增殖、集落形成、侵袭和迁移，并促进细胞凋亡。hsa_circ_0004712 可直接靶向 miR-331-3p，其抑制剂可逆转 hsa_circ_0004712 下调的作用。FZD4 是 miR-331-3p 的靶基因，hsa_circ_0004712 可通过靶向 miR-331-3p 正向调节 FZD4 表达。miR-331-3p 恢复的抗肿瘤作用可被 FZD4 过表达逆转。下调 hsa_circ_0004712 还通过调节 miR-331-3p 和 FZD4 抑制体内肿瘤的发展。
综上所述，hsa_circ_0004712 缺失通过介导 miR-331-3p/FZD4 通路抑制 OC 发展，表明 hsa_circ_0004712 可能是 OC 诊断和治疗的有前途的生物标志物。
