Department of Psychiatry and Human Behavior, Alpert Medical School of Brown University, Providence, RI, USA.
Department of Pediatrics, Women and Infants Hospital of Rhode Island, 101 Dudley Street, Providence, RI, 02905, USA.
Clin Epigenetics. 2021 Sep 10;13(1):171. doi: 10.1186/s13148-021-01164-9.
Prenatal risk factors are related to poor health and developmental outcomes for infants, potentially via epigenetic mechanisms. We tested associations between person-centered prenatal risk profiles, cumulative prenatal risk models, and epigenome-wide DNA methylation (DNAm) in very preterm neonates.
We studied 542 infants from a multi-center study of infants born < 30 weeks postmenstrual age. We assessed 24 prenatal risk factors via maternal report and medical record review. Latent class analysis was used to define prenatal risk profiles. DNAm was quantified from neonatal buccal cells using the Illumina MethylationEPIC Beadarray.
We identified three latent profiles of women: a group with few risk factors (61%) and groups with elevated physical (26%) and psychological (13%) risk factors. Neonates born to women in higher risk subgroups had differential DNAm at 2 CpG sites. Higher cumulative prenatal risk was associated with methylation at 15 CpG sites, 12 of which were located in genes previously linked to physical and mental health and neurodevelopment.
We observed associations between prenatal risk factors and DNAm in very preterm infants using both person-centered and cumulative risk approaches. Epigenetics offers a potential biological indicator of prenatal risk exposure.
产前风险因素与婴儿的健康和发育结果不良有关，这可能是通过表观遗传机制实现的。我们检测了个体中心的产前风险概况、累积产前风险模型与极早产儿全基因组 DNA 甲基化（DNAm）之间的关联。
我们研究了来自一个多中心研究的 542 名胎龄小于 30 周的婴儿。我们通过母亲报告和病历回顾评估了 24 个产前风险因素。采用潜在类别分析定义产前风险概况。使用 Illumina MethylationEPIC BeadArray 从新生儿口腔细胞中定量 DNAm。
我们确定了三种女性的潜在风险概况：一组风险因素较少（61%），另一组存在较高的身体（26%）和心理（13%）风险因素。来自高风险亚组女性的新生儿在 2 个 CpG 位点存在差异的 DNAm。较高的累积产前风险与 15 个 CpG 位点的甲基化有关，其中 12 个位于先前与身心健康和神经发育相关的基因中。
我们使用个体中心和累积风险方法观察到极早产儿的产前风险因素与 DNAm 之间的关联。表观遗传学提供了产前风险暴露的潜在生物学指标。
