Department of Biology, Faculty of Science and Art, Giresun University, Giresun, Turkey.
Department of Medical Services and Techniques, Vocational School of Health Services, Giresun University, Giresun, Turkey.
Sci Rep. 2022 Aug 25;12(1):14471. doi: 10.1038/s41598-022-18791-8.
In this study, AFM toxicity and the protective role of trans-resveratrol (t-rsv) against this toxicity were investigated with the help of multiple parameters in albino mice. As a result, AFM (16 mg/kg b.w) administration caused a decrease in body, kidney and liver weights. This reduction was associated with a decrease in feed consumption. AFM induced an increase in AST and ALT enzyme parameters and BUN, creatinine and MDA levels and a decrease in GSH levels. These increases have been associated with liver and kidney cell damage. AFM decreased MI and encouraged increases in MN and CAs numbers. The decrease in MI was correlated with AFM1-tubulin and the increase in CAs was associated with the AFM-DNA interaction, which was demonstrated by molecular docking and spectral shifting. Besides, the decrease in DNA damage and amount was demonstrated by the comet assay technique. Administration of t-rsv (10 and 20 mg/kg b.w) reduced the toxic effects of AFM and caused a dose-dependent improvement in all physiological, biochemical and cytogenetic parameter values studied. For this reason, foods containing t-rsv or food supplements should be consumed in the daily diet to reduce the effect of toxic agents.
在这项研究中，借助于白化小鼠的多个参数，研究了原子力显微镜（AFM）的毒性以及反式白藜芦醇（t-rsv）对这种毒性的保护作用。结果表明，AFM（16mg/kg b.w）给药导致体重、肾脏和肝脏重量减轻。这种减少与饲料消耗减少有关。AFM 诱导 AST 和 ALT 酶参数以及 BUN、肌酐和 MDA 水平升高，GSH 水平降低。这些增加与肝和肾细胞损伤有关。AFM 降低了 MI 并鼓励 MN 和 CAs 数量的增加。MI 的减少与 AFM1-微管蛋白有关，而 CAs 的增加与 AFM-DNA 相互作用有关，这通过分子对接和光谱移位得到了证明。此外，彗星试验技术证明了 DNA 损伤和数量的减少。t-rsv（10 和 20mg/kg b.w）的给药减轻了 AFM 的毒性作用，并使所有研究的生理、生化和细胞遗传学参数值呈剂量依赖性改善。因此，应在日常饮食中摄入含有 t-rsv 的食物或食品补充剂，以减少有毒物质的影响。
