Wang Yuanyong, Ma Minge, Li Chuan, Yang Yuling, Wang Maolong
Department of Thoracic Surgery, The Affiliated Hospital of Qingdao University, Qingdao, China.
Department of Radiology, The Affiliated Hospital of Qingdao University, Qingdao, China.
Front Oncol. 2021 Aug 26;11:645771. doi: 10.3389/fonc.2021.645771. eCollection 2021.
GAS6 antisense RNA 1 (GAS6-AS1) is a long non-coding RNA involved in hepatocellular carcinoma and gastric cancer. However, the functional role of GAS6-AS1 in lung adenocarcinoma (LUAD) remains unclear. In the present study, qRT-PCR was used to measure the levels of GAS6-AS1, GIMAP6 and miR-24-3p expression in LUAD samples and cell lines. CCK-8 and colony formation assays were used to determine cell proliferation. Cell migration and invasion were evaluated using wound healing and transwell assays, respectively. The potential interactions between molecules were assessed using RNA immunoprecipitation and luciferase reporter assays. Western blot analysis was used to quantify protein expression. The anti-tumor effect of over-expressed GAS6-AS1 on LUAD was also examined in xenograft tumor experiments. The expression of GAS6-AS1 was notably downregulated in LUAD samples and cell lines and associated with a poor prognosis. GAS6-AS1 overexpression inhibited the migration and invasion of A549 and H1650 cells. Down-expressed GAS6-AS1 acted as a sponge for miR-24-3p and down-regulated the expression of its target, GTPase IMAP Family Member 6. These findings suggested that GAS6-AS1 might represent a potential diagnostic biomarker for LUAD.
生长停滞特异性蛋白6反义RNA 1（GAS6-AS1）是一种长链非编码RNA，与肝细胞癌和胃癌有关。然而，GAS6-AS1在肺腺癌（LUAD）中的功能作用仍不清楚。在本研究中，采用qRT-PCR检测LUAD样本和细胞系中GAS6-AS1、GIMAP6和miR-24-3p的表达水平。采用CCK-8和集落形成试验来确定细胞增殖。分别使用伤口愈合试验和Transwell试验评估细胞迁移和侵袭。使用RNA免疫沉淀和荧光素酶报告基因试验评估分子之间的潜在相互作用。采用蛋白质印迹分析来定量蛋白质表达。在异种移植肿瘤实验中也检测了过表达GAS6-AS1对LUAD的抗肿瘤作用。GAS6-AS1在LUAD样本和细胞系中的表达明显下调，且与预后不良相关。GAS6-AS1过表达抑制了A549和H1650细胞的迁移和侵袭。低表达的GAS6-AS1作为miR-24-3p的海绵，下调其靶标GTP酶IMAP家族成员6的表达。这些发现表明，GAS6-AS1可能是LUAD的一种潜在诊断生物标志物。
