Hu Wanxue, Hu Xiaoyi, Zhu Yongchao, Li Min, Meng Hongyu, Zhao Hongbo
Department of Clinical Laboratory, Hua County People's Hospital, Anyang, China.
Department of Nursing, Henan Health Cadre College, Zhengzhou, China.
Transl Cancer Res. 2024 Oct 31;13(10):5190-5201. doi: 10.21037/tcr-24-833. Epub 2024 Oct 29.
Genetic alterations have important roles in cancer development and progression. (spindle and kinetochore associated complex subunit 2) is a mitotic component that plays a critical role in maintaining the silence of the metaphase plate and spindle checkpoint. However, the exact role of in hepatocellular carcinoma (HCC) remains unclear. The current study aimed to comprehensively identify the function of in HCC.
We utilized various databases and bioinformatics tools, such as The Cancer Genome Atlas (TCGA), survminer package, Tumor Immune Estimation Resource (TIMER), cBioPortal website, clusterProfiler package, gene set enrichment analysis (GSEA), miRWalk, TargetScanHuman8.0, miRDB, DIANA and Cytoscape to identify the role of in HCC.
Our results showed that patients with HCC exhibited a high expression. Further, the high expression group had a worse overall survival (OS). And was associated with tumor stage and the immune system. In addition, 188 co-expression genes of participated in some processes including cell cycle, DNA replication and so on. The tumor had a lower hsa-miR-19b-1-5p and hsa-miR-378a-5p expression, and these two microRNAs (miRNAs) were also correlated with OS. SNHG14, SNHG15, and SPCA6P-AS were significantly negatively correlated with hsa-378a-5p, and these three long non-coding RNAs (lncRNAs) showed a positive correlation with (P<0.05). is a member of competing endogenous RNA (ceRNA). Moreover, it is related to SPACA6P-AS/hsa-miR-378a-5p/, SNHG14/hsa-miR-378a-5p/, and SNHG15/hsa-miR-378a-5p/, which play significant roles in tumor progression.
is associated with OS, tumor stage, and immune infiltrating cells in HCC. Thus, we propose that functions as a ceRNA and influences tumorigenesis. These findings lay the foundation for future research in the field of HCC.
基因改变在癌症的发生和发展中起着重要作用。（纺锤体和动粒相关复合体亚基2）是一种有丝分裂成分，在维持中期板和纺锤体检查点的沉默中起关键作用。然而，其在肝细胞癌（HCC）中的确切作用仍不清楚。本研究旨在全面鉴定其在HCC中的功能。
我们利用了各种数据库和生物信息学工具，如癌症基因组图谱（TCGA）、survminer软件包、肿瘤免疫估计资源（TIMER）、cBioPortal网站、clusterProfiler软件包、基因集富集分析（GSEA）、miRWalk、TargetScanHuman8.0、miRDB、DIANA和Cytoscape来鉴定其在HCC中的作用。
我们的结果显示，HCC患者表现出高表达。此外，高表达组的总生存期（OS）较差。并且其与肿瘤分期和免疫系统有关。此外，的188个共表达基因参与了包括细胞周期、DNA复制等一些过程。肿瘤中hsa-miR-19b-1-5p和hsa-miR-378a-5p表达较低，这两种微小RNA（miRNA）也与OS相关。SNHG14、SNHG15和SPCA6P-AS与hsa-378a-5p显著负相关，这三种长链非编码RNA（lncRNA）与呈正相关（P<0.05）。是竞争性内源RNA（ceRNA）的成员。此外，它与SPACA6P-AS/hsa-miR-378a-5p/、SNHG14/hsa-miR-378a-5p/和SNHG15/hsa-miR-378a-5p/有关，它们在肿瘤进展中起重要作用。
与HCC的OS、肿瘤分期和免疫浸润细胞有关。因此，我们提出其作为ceRNA发挥作用并影响肿瘤发生。这些发现为HCC领域的未来研究奠定了基础。
