The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, 710049, China; College of Bioresources and Materials Engineering, Shaanxi University of Science & Technology, Xi'an, 710021, China.
Department of Burns and Cutaneous Surgery, Xijing Hospital, The Fourth Military Medical University, Xi 'an, 710032, China.
Talanta. 2021 Dec 1;235:122719. doi: 10.1016/j.talanta.2021.122719. Epub 2021 Jul 15.
Here, a dual lock-and-key fluorescence probe was developed for visualizing the inflammatory process in myocardial H9C2 cells. The probe possessed two-photon properties, viscosity sensitivity, and hydrogen peroxide (HO) responsiveness. A thiocarbamate spacer between fluorophore and HO responsive unit enabled the release of carbonyl sulfide (COS). This rapidly converts to the anti-inflammatory hydrogen sulfide (HS) by the ubiquitous enzyme carbon anhydrase. The probe displayed a dual response towards hydrogen peroxide and viscosity in vitro. No obvious fluorescence changes were observed towards either hydrogen peroxide or viscosity alone. In cellular experiments, the probe demonstrated good biocompatibility, low toxicity, and was shown responses towards exogenous and endogenous hydrogen peroxide under viscosity conditions. LPS induced cell inflammation showed it was able to effectively alleviate the inflammation-caused damage by releasing HS and eliminating HO. The new protocol demonstrates its promising to achieve diagnosis and treatment of cellular inflammatory process.
这里，开发了一种双锁钥荧光探针用于可视化心肌 H9C2 细胞中的炎症过程。该探针具有双光子性质、粘度敏感性和过氧化氢（HO）响应性。荧光团和 HO 响应单元之间的硫代氨基甲酸酯间隔物能够释放碳酰硫（COS）。这通过普遍存在的酶碳酸酐酶迅速转化为抗炎性的硫化氢（HS）。探针在体外对过氧化氢和粘度表现出双重响应。单独的过氧化氢或粘度都没有观察到明显的荧光变化。在细胞实验中，探针表现出良好的生物相容性、低毒性，并且在粘度条件下对外源和内源性过氧化氢表现出响应。LPS 诱导的细胞炎症表明，它能够通过释放 HS 和消除 HO 来有效缓解炎症引起的损伤。该新方案有望实现细胞炎症过程的诊断和治疗。
