Kangwan Napapan, Pintha Komsak, Khanaree Chakkrit, Kongkarnka Sarawut, Chewonarin Teera, Suttajit Maitree
Division of Physiology, School of Medical Sciences, University of Phayao, Phayao, Thailand.
Division of Biochemistry, School of Medical Sciences, University of Phayao, Phayao, Thailand.
Res Pharm Sci. 2021 Aug 19;16(5):464-473. doi: 10.4103/1735-5362.323913. eCollection 2021 Oct.
Ulcerative colitis is a chronic inflammatory bowel disease that involves diffused inflammation of the large intestine. Omega-3 fatty acid (FA) has been known to regulate the inflammatory response associated with ulcerative colitis pathogenesis. is a valuable source of omega-3 FA and α-linolenic acid (ALA) contained in its seed oil. Therefore, the aim of this study was to evaluate the anti-inflammatory effect of seed oil (PSO) on colitis induced by dextran sulfate sodium (DSS) in a mouse model.
PSO was extracted using a cold-pressed extractor and FA composition of PSO was analyzed by GC-MS. Acute colitis in mice was induced with 3% DSS in drinking water for 7 days. Some mice were treated with PSO (20, 100, 200 mg/kg BW) for 3 weeks before the DSS administration. Sulfasalazine was used as a positive control. The clinical features, histopathologic, serum, and gene expression of proinflammatory cytokines in the colon were assessed.
FINDING/RESULTS: PSO contained the highest proportion of ALA (61.51%). Furthermore, PSO pretreatment evidently reduced body weight loss, diminished diarrhea, gross bleeding, and DSS-induced colon shortening. PSO pretreatment attenuated histopathological changes in response to DSS-induced colitis. PSO pretreatment also markedly decreased inflammatory response in serum and the colon tissue of DSS-induced mice.
ALA in PSO is suggested to be mainly responsible for the reduction of DSS-induced colitis through suppressing inflammatory markers. PSO could be further developed as a functional health supplement, which would be beneficial for anti-inflammation in the colonic mucosa.
溃疡性结肠炎是一种慢性炎症性肠病，累及大肠的弥漫性炎症。已知ω-3脂肪酸（FA）可调节与溃疡性结肠炎发病机制相关的炎症反应。 是ω-3 FA和其种子油中所含α-亚麻酸（ALA）的宝贵来源。因此，本研究的目的是评估种子油（PSO）对葡聚糖硫酸钠（DSS）诱导的小鼠结肠炎的抗炎作用。
使用冷压提取器提取PSO，并通过气相色谱-质谱联用仪（GC-MS）分析PSO的脂肪酸组成。用3% DSS饮用水诱导小鼠急性结肠炎7天。一些小鼠在给予DSS前3周用PSO（20、100、200 mg/kg体重）治疗。柳氮磺胺吡啶用作阳性对照。评估结肠的临床特征、组织病理学、血清和促炎细胞因子的基因表达。
发现/结果：PSO中ALA的比例最高（61.51%）。此外，PSO预处理明显减轻体重减轻、减少腹泻、大出血和DSS诱导的结肠缩短。PSO预处理减轻了对DSS诱导的结肠炎的组织病理学变化。PSO预处理还显著降低了DSS诱导小鼠血清和结肠组织中的炎症反应。
PSO中的ALA被认为主要通过抑制炎症标志物来减轻DSS诱导的结肠炎。PSO可进一步开发为功能性健康补充剂，这将有利于结肠黏膜的抗炎作用。
