Park Deung Dae, Yum Hye-Won, Zhong Xiancai, Kim Seung Hyeon, Kim Seong Hoon, Kim Do-Hee, Kim Su-Jung, Na Hye-Kyung, Sato Atsuya, Miura Takehito, Surh Young-Joon
Tumor Microenvironment Global Core Research Center, Seoul National UniversitySeoul, South Korea.
Department of Molecular Medicine and Biopharmaceutical Sciences, College of Pharmacy, Seoul National UniversitySeoul, South Korea.
Front Pharmacol. 2017 Aug 8;8:482. doi: 10.3389/fphar.2017.00482. eCollection 2017.
is a culinary and medicinal herb which has a strong anti-inflammatory and antioxidative effects. In the present study, we investigated the effects of extract (PE) against dextran sulfate sodium (DSS)-induced mouse colitis, an animal model that mimics human inflammatory bowel disease (IBD). Five-week-old male ICR mice were treated with a daily dose of PE (20 or 100 mg/kg, ) for 1 week, followed by administration of 3% DSS in double distilled drinking water and PE by gavage for another week. DSS-induced colitis was characterized by body weight loss, colon length shortening, diarrhea and bloody stool, and these symptoms were significantly ameliorated by PE treatment. PE administration suppressed DSS-induced expression of proinflammatory enzymes, including cyclooxygenase-2 and inducible nitric oxide synthase as well as cyclin D1, in a dose-dependent fashion. Nuclear factor-kappa B (NF-κB) and signal transducer and activator of transcription 3 (STAT3) are major transcriptional regulators of inflammatory signaling. PE administration significantly inhibited the activation of both NF-κB and STAT3 induced by DSS, while it elevated the accumulation of Nrf2 and heme oxygenase-1 in the colon. In another experiment, treatment of CCD841CoN human normal colon epithelial cells with PE (10 mg/ml) resulted in the attenuation of the tumor necrosis factor-α-induced expression/activation of mediators of proinflammatory signaling. The above results indicate that PE has a preventive potential for use in the management of IBD.
是一种具有很强抗炎和抗氧化作用的烹饪及药用草本植物。在本研究中，我们研究了[提取物名称]提取物（PE）对葡聚糖硫酸钠（DSS）诱导的小鼠结肠炎的影响，该动物模型模拟人类炎症性肠病（IBD）。5周龄雄性ICR小鼠每日给予PE（20或100mg/kg，[给药途径]），持续1周，随后在双蒸饮用水中给予3% DSS，并通过灌胃给予PE，持续1周。DSS诱导的结肠炎表现为体重减轻、结肠长度缩短、腹泻和便血，而PE治疗可显著改善这些症状。PE给药以剂量依赖的方式抑制DSS诱导的促炎酶表达，包括环氧合酶-2、诱导型一氧化氮合酶以及细胞周期蛋白D1。核因子-κB（NF-κB）和信号转导及转录激活因子3（STAT3）是炎症信号的主要转录调节因子。PE给药显著抑制DSS诱导的NF-κB和STAT3的激活，同时提高结肠中Nrf2和血红素加氧酶-1的积累。在另一项实验中，用PE（10mg/ml）处理CCD841CoN人正常结肠上皮细胞可减轻肿瘤坏死因子-α诱导的促炎信号介质的表达/激活。上述结果表明，PE在IBD的管理中具有预防潜力。
