Adjei Silas, Amponsah Isaac Kingsley, Bekoe Samuel Oppong, Harley Benjamin Kingsley, Mensah Kwesi Boadu, Mensah Abraham Yeboah, Baah Michael Kwesi, Fosu-Mensah Gabriel
Department of Herbal Medicine, Faculty of Pharmacy and Pharmaceutical Sciences, College of Health Sciences, KNUST, Kumasi, Ghana.
Department of Pharmacognosy, Faculty of Pharmacy and Pharmaceutical Sciences, College of Health Sciences, KNUST, Kumasi, Ghana.
Heliyon. 2021 Sep 2;7(9):e07910. doi: 10.1016/j.heliyon.2021.e07910. eCollection 2021 Sep.
Sweet fruit, an under-utilised crop specie of Ghana, has not been validated for its ethnomedical use in managing inflammatory conditions. Therefore, the study sought to investigate its anti-inflammatory and antioxidant activities as well as isolate and quantify one of its active constituents.
anti-inflammatory activity of the methanol fruit extract was evaluated using the carrageenan-induced oedema model in chicks. The antioxidant property was also investigated using the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. The acute and subacute toxicity studies of the fruit extract were evaluated in rodent models.
No signs of autonomic and central nervous system stimulation/depression were recorded. The LD by oral route, was estimated to be beyond 3000 mg/kg. Subacute studies revealed an increase in red blood cell and lymphocyte counts. Liver enzymes, serum proteins and bilirubin levels did not significantly increase. The crude extracts at doses of 10, 30 and 100 mg/kg inhibited paw oedema considerably. The ethyl acetate fraction showed the highest antioxidant activity (IC = 99.35 ± 0.77 μg/mL). Oleanolic acid, isolated from the ethyl acetate extract, showed significant anti-inflammatory and antioxidant activities. A sensitive high-performance liquid chromatography method for the detection and estimation of oleanolic acid, as a biomarker compound for fruit, was developed and validated for quality assurance purposes.
The extract of fruits possesses considerable anti-inflammatory and antioxidant properties and was non-toxic under laboratory conditions.
甜果是加纳一种未得到充分利用的作物品种，其在治疗炎症方面的民族医学用途尚未得到验证。因此，本研究旨在调查其抗炎和抗氧化活性，并分离和定量其一种活性成分。
使用角叉菜胶诱导的雏鸡水肿模型评估甲醇果实提取物的抗炎活性。还使用2,2-二苯基-1-苦基肼（DPPH）自由基清除试验研究了其抗氧化性能。在啮齿动物模型中评估了果实提取物的急性和亚急性毒性研究。
未记录到自主神经系统和中枢神经系统刺激/抑制的迹象。经口途径的半数致死量估计超过3000mg/kg。亚急性研究显示红细胞和淋巴细胞计数增加。肝酶、血清蛋白和胆红素水平没有显著升高。10、30和100mg/kg剂量的粗提物对爪部水肿有显著抑制作用。乙酸乙酯部分显示出最高的抗氧化活性（IC = 99.35±0.77μg/mL）。从乙酸乙酯提取物中分离出的齐墩果酸具有显著的抗炎和抗氧化活性。开发并验证了一种灵敏的高效液相色谱法，用于检测和定量齐墩果酸，作为甜果的生物标志物化合物，以确保质量。
甜果提取物具有相当的抗炎和抗氧化特性，在实验室条件下无毒。
