Thathola Pooja, Agnihotri Vasudha, Pandey Anita
Centre for Land and Water Resource Management, G. B. Pant National Institute of Himalayan Environment, Kosi-Katarmal, Almora, Uttarakhand, 263643, India.
Department of Biotechnology, Graphic Era University, Bell Road, Clement Town, Dehradun, Uttarakhand, 248002, India.
Curr Microbiol. 2021 Nov;78(11):3924-3935. doi: 10.1007/s00284-021-02644-0. Epub 2021 Sep 14.
Caffeine, a xenobiotic compound, is continuously released into the environment. Fifteen psychrotolerant bacterial strains, isolated from the Indian Himalayan region, were screened for their caffeine degradation capacity. The medium for the growth of bacteria was optimized using Box-Behnken method. Among these bacteria, Pseudomonassp. (GBPI_Hb5), showing the best response, was further used for caffeine degradation in batch mode. The culture medium, having caffeine as a sole source of carbon, was used for analyzing the effect of pH, agitation speed, temperature, inoculum volume, and caffeine concentration on bacterial growth and its caffeine degradation potential. The bacterium GBPI_Hb5 showed approx. 93% caffeine degradation up to 96 h under controlled conditions. The compounds produced during the degradation of caffeine were also studied. The study is likely to have implications in the bioremediation of caffeine from polluted environments.
咖啡因作为一种外源性化合物，正不断释放到环境中。从印度喜马拉雅地区分离出15株耐冷细菌菌株，并对其咖啡因降解能力进行了筛选。使用Box-Behnken方法优化了细菌生长培养基。在这些细菌中，表现出最佳反应的假单胞菌（GBPI_Hb5）进一步用于分批模式下的咖啡因降解。以咖啡因作为唯一碳源的培养基用于分析pH值、搅拌速度、温度、接种量和咖啡因浓度对细菌生长及其咖啡因降解潜力的影响。在受控条件下，细菌GBPI_Hb5在96小时内显示出约93%的咖啡因降解率。还研究了咖啡因降解过程中产生的化合物。该研究可能对污染环境中咖啡因的生物修复具有重要意义。
