Estes Bradley T, Enomoto Masataka, Moutos Franklin T, Carson Megan A, Toth Jeffrey M, Eggert Peter, Stallrich Jonathan, Willard Vincent P, Veis Deborah J, Little Dianne, Guilak Farshid, Lascelles B Duncan X
Cytex Therapeutics Inc., Durham, NC, USA.
Translational Research in Pain Program, Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA.
Sci Adv. 2021 Sep 17;7(38):eabi5918. doi: 10.1126/sciadv.abi5918. Epub 2021 Sep 15.
Articular cartilage has unique load-bearing properties but has minimal capacity for intrinsic repair. Here, we used three-dimensional weaving, additive manufacturing, and autologous mesenchymal stem cells to create a tissue-engineered, bicomponent implant to restore hip function in a canine hip osteoarthritis model. This resorbable implant was specifically designed to function mechanically from the time of repair and to biologically integrate with native tissues for long-term restoration. A massive osteochondral lesion was created in the hip of skeletally mature hounds and repaired with the implant or left empty (control). Longitudinal outcome measures over 6 months demonstrated that the implant dogs returned to normal preoperative values of pain and function. Anatomical structure and functional biomechanical properties were also restored in the implanted dogs. Control animals never returned to normal and exhibited structurally deficient repair. This study provides clinically relevant evidence that the bicomponent implant may be a potential therapy for moderate hip osteoarthritis.
关节软骨具有独特的承重特性，但自身修复能力极小。在此，我们利用三维编织、增材制造和自体间充质干细胞来创建一种组织工程双组分植入物，以在犬髋关节骨关节炎模型中恢复髋关节功能。这种可吸收植入物经过专门设计，从修复之时起就能发挥机械功能，并与天然组织进行生物整合以实现长期修复。在骨骼成熟的猎犬髋关节中制造一个大面积的骨软骨损伤，并用植入物进行修复或保持空缺（作为对照）。超过6个月的纵向结果测量表明，植入植入物的犬只恢复到术前疼痛和功能的正常水平。植入犬只的解剖结构和功能生物力学特性也得到了恢复。对照动物从未恢复正常，并且表现出结构上有缺陷的修复。这项研究提供了临床相关证据，表明双组分植入物可能是治疗中度髋关节骨关节炎的一种潜在疗法。
