Schulich Faculty of Chemistry, Technion-Israel Institute of Technology Haifa, 3200008, Israel.
Faculty of Biology, Technion-Israel Institute of Technology Haifa, 3200008, Israel.
Chem Commun (Camb). 2021 Sep 16;57(74):9438-9441. doi: 10.1039/d1cc04045f.
Protein post-translational modifications are involved in essentially all aspects of cellular signaling. Their dynamic nature and the difficulties in installing them using enzymatic approaches limits their direct study in human cells. Reported herein is the first synthesis, delivery and cellular study of a stable phosphoubiquitin probe. Our results compare Parkin's substrate preference during mitophagy direct visualization of a phosphorylated ubiquitin probe in the cellular environment.
蛋白质翻译后修饰参与细胞信号转导的各个方面。由于其动态性质以及使用酶法进行修饰的困难，限制了对其在人细胞中的直接研究。本文首次报道了一种稳定的磷酸泛素探针的合成、递送和细胞研究。我们的结果比较了 Parkin 在有丝分裂自噬过程中的底物偏好，直接观察到细胞环境中磷酸化泛素探针的存在。
