Keck Graduate Institute, The Claremont Colleges, Claremont, CA, 91711, USA; Cardea Bio, San Diego, CA, 92121, USA.
Cardea Bio, San Diego, CA, 92121, USA.
Biosens Bioelectron. 2022 Jan 1;195:113605. doi: 10.1016/j.bios.2021.113605. Epub 2021 Sep 8.
As biological research has synthesized genomics, proteomics, metabolomics, and transcriptomics into systems biology, a new multiomics approach to biological research has emerged. Today, multiomics studies are challenging and expensive. An experimental platform that could unify the multiple omics approaches to measurement could increase access to multiomics data by enabling more individual labs to successfully attempt multiomics studies. Field effect biosensing based on graphene transistors have gained significant attention as a potential unifying technology for such multiomics studies. This review article highlights the outstanding performance characteristics that makes graphene field effect transistor an attractive sensing platform for a wide variety of analytes important to system biology. In addition to many studies demonstrating the biosensing capabilities of graphene field effect transistors, they are uniquely suited to address the challenges of multiomics studies by providing an integrative multiplex platform for large scale manufacturing using the well-established processes of semiconductor industry. Furthermore, the resulting digital data is readily analyzable by machine learning to derive actionable biological insight to address the challenge of data compatibility for multiomics studies. A critical stage of systems biology will be democratizing multiomics study, and the graphene field effect transistor is uniquely positioned to serve as an accessible multiomics platform.
随着生物研究将基因组学、蛋白质组学、代谢组学和转录组学综合成系统生物学，一种新的多组学方法应运而生。如今，多组学研究具有挑战性和昂贵。如果有一种实验平台能够将多种组学方法统一到测量中，通过使更多的个体实验室能够成功地尝试多组学研究，就可以增加对多组学数据的访问。基于石墨烯晶体管的场效应生物传感已经引起了人们的极大关注，因为它可能是一种统一的多组学研究技术。本文综述突出了石墨烯场效应晶体管出色的性能特点，使其成为系统生物学中许多重要分析物的有吸引力的传感平台。除了许多研究证明了石墨烯场效应晶体管的生物传感能力外，它们还通过提供使用半导体行业成熟工艺的大规模制造的集成多路复用平台，特别适合解决多组学研究的挑战。此外，所得到的数字数据可以通过机器学习进行分析，以便从数据兼容性的角度为多组学研究提供可操作的生物学见解。系统生物学的一个关键阶段将是使多组学研究民主化，而石墨烯场效应晶体管则是作为一种易于使用的多组学平台的独特定位。
