Yang Xiaoke, Chen Bo, Zhang Mingyue, Xu Shengqian, Shuai Zongwen
Department of Rheumatology and Immunology, The First Affiliated Hospital of Anhui Medical University, Hefei, China.
Department of Nuclear Medicine, Chaohu Hospital Affiliated to Anhui Medical University, Hefei, China.
Front Pharmacol. 2021 Sep 1;12:728015. doi: 10.3389/fphar.2021.728015. eCollection 2021.
To observe and evaluate the correlation between single-nucleotide polymorphisms (SNPs) and messenger RNA (mRNA) level related to tristetraprolin (TTP) in Chinese rheumatoid arthritis (RA). TapMan SNP was used for genotyping analysis in 580 RA patients and 554 healthy people. Association between gene polymorphisms (rs251864 and rs3746083) and RA was obtained. Quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) technology was applied for the detection of TTP mRNA level in peripheral blood mononuclear cells (PBMCs) in 36 RA patients and 37 healthy people. We observed that the allele T of rs3746083 increased RA susceptibility ( = 0.019). A significant difference was found under the dominant model of rs3746083 ( = 0.037). Further analysis showed the allele distribution of rs3746083 was nominally correlated with RF phenotype of RA patients ( = 0.045). Nevertheless, the association between rs251864 and the incidence of RA was no statistically significant ( > 0.05). The TTP expression level in PBMCs of RA patients was significantly reduced ( < 0.001). In conclusion, the results of this experiment support that TTP may be involved in the pathogenesis of RA.
观察并评估中国类风湿关节炎（RA）患者中与锌指蛋白36（TTP）相关的单核苷酸多态性（SNP）与信使核糖核酸（mRNA）水平之间的相关性。采用TaqMan SNP技术对580例RA患者和554例健康人进行基因分型分析，得出基因多态性（rs251864和rs3746083）与RA之间的关联。应用定量实时逆转录聚合酶链反应（qRT-PCR）技术检测36例RA患者和37例健康人外周血单个核细胞（PBMC）中TTP mRNA水平。我们观察到rs3746083的T等位基因增加了RA易感性（P = 0.019）。在rs3746083的显性模型下发现有显著差异（P = 0.037）。进一步分析表明，rs3746083的等位基因分布与RA患者的类风湿因子（RF）表型呈名义上的相关性（P = 0.045）。然而，rs251864与RA发病率之间的关联无统计学意义（P > 0.05）。RA患者PBMC中TTP表达水平显著降低（P < 0.001）。总之，本实验结果支持TTP可能参与RA的发病机制。
