School of Business and Trade, Nanjing Vocational University of Industry Technology, Nanjing, 210046, China.
Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, College of Biology and the Environment, Nanjing Forestry University, Nanjing, 210037, China.
Mol Biol Rep. 2021 Nov;48(11):7113-7125. doi: 10.1007/s11033-021-06702-5. Epub 2021 Sep 19.
An ornamental plant often seen in gardens and farmhouses, Musa basjoo Siebold can also be used as Chinese herbal medicine. Its pseudostem and leaves are diuretic; its root can be decocted together with ginger and licorice to cure gonorrhea and diabetes; the decoct soup of its pseudostem can help relieve heat, and the decoct soup of its dried flower can treat cerebral hemorrhage. There have not been many chloroplast genome studies on M. basjoo Siebold.
We characterized its complete chloroplast genome using Novaseq 6000 sequencing. This paper shows that the length of the chloroplast genome M. basjoo Siebold is 172,322 bp, with 36.45% GC content. M. basjoo Siebold includes a large single-copy region of 90,160 bp, a small single-copy region of 11,668 bp, and a pair of inverted repeats of 35,247 bp. Comparing the genomic structure and sequence data of closely related species, we have revealed the conserved gene order of the IR and LSC/SSC regions, which has provided a very inspiring discovery for future phylogenetic research.
Overall, this study has constructed an evolutionary tree of the genus Musa species with the complete chloroplast genome sequence for the first time. As can be seen, there is no obvious multi-branching in the genus, and M. basjoo Siebold and Musa itinerans are the closest relatives.
芭蕉是一种常见于花园和农舍的观赏植物，也可用作中草药。其假茎和叶片有利尿作用；其根可与生姜和甘草一起煎服，治疗淋病和糖尿病；其假茎煎剂可清热，干花煎剂可治疗脑出血。目前对芭蕉属植物的叶绿体基因组研究较少。
我们使用 Novaseq 6000 测序技术对其完整的叶绿体基因组进行了表征。本文表明，芭蕉属植物的叶绿体基因组长度为 172322bp，GC 含量为 36.45%。芭蕉属植物包含一个 90160bp 的大单拷贝区、一个 11668bp 的小单拷贝区和一对 35247bp 的反向重复区。通过比较近缘物种的基因组结构和序列数据，揭示了 IR 和 LSC/SSC 区的保守基因顺序，为未来的系统发育研究提供了非常有启发性的发现。
总的来说，本研究首次构建了一个包含完整叶绿体基因组序列的芭蕉属物种进化树。可以看出，属内没有明显的多分支，芭蕉和旅行蕉是最接近的亲缘关系。
