Department of Ophthalmology, Nancy University Hospital, Vandœuvre-lès-Nancy, France; Institut de la Vision, Sorbonne Universités, UPMC Univ Paris 06, Inserm, CNRS, 17, rue Moreau, 75012 Paris, France.
Institut de la Vision, Sorbonne Universités, UPMC Univ Paris 06, Inserm, CNRS, 17, rue Moreau, 75012 Paris, France.
J Fr Ophtalmol. 2021 Nov;44(9):1349-1357. doi: 10.1016/j.jfo.2021.04.007. Epub 2021 Sep 17.
To compare the expression profiles of various cytokines and chemokines in vitreous samples from patients with retinal detachment (RD) to those from controls and to analyze their association with various clinical features.
In this prospective study, undiluted vitreous fluid was obtained from 41 patients with primary RD and 33 controls with macular hole or vitreomacular traction. A multiplex bead immunoassay was performed to determine the expression of 27 inflammatory mediators.
Eleven mediators were significantly upregulated in the vitreous of RD patients compared with controls, including the following: cytokines IL-1ra, IL-6, IL-7, IL-8, IFN-γ; chemokines CCL2, CCL3, CCL4, CXCL10 and CCL11 and growth factor G-CSF. Correlation analyses showed that levels of IL-1ra, CXCL10, CCL11 and G-CSF were positively correlated to the extent of detachment, while those of IL-1ra and CXCL10 were associated with the duration of detachment. There was also a positive association between the concentrations of CXCL10 and CCL11 and preoperative flare values. Additional analysis revealed that flare values and both CXCL10 and CCL11 levels were significantly higher in eyes with grade B or C proliferative vitreoretinopathy (PVR).
Our results confirm that RD induces a marked inflammatory response with a complex cytokine network. We identified proteins specifically linked to several clinical features that might contribute to photoreceptor degeneration and PVR-related redetachment. These proteins may represent potential therapeutic targets for improving the anatomical and functional outcomes of RD surgery.
比较视网膜脱离（RD）患者和对照组患者玻璃体样本中各种细胞因子和趋化因子的表达谱，并分析它们与各种临床特征的关系。
在这项前瞻性研究中，从 41 例原发性 RD 患者和 33 例黄斑裂孔或玻璃体牵引对照组患者中获得未稀释的玻璃体。采用多重珠免疫分析测定 27 种炎症介质的表达。
与对照组相比，RD 患者的玻璃体中有 11 种介质明显上调，包括以下细胞因子：IL-1ra、IL-6、IL-7、IL-8、IFN-γ；趋化因子 CCL2、CCL3、CCL4、CXCL10 和 CCL11 以及生长因子 G-CSF。相关分析表明，IL-1ra、CXCL10、CCL11 和 G-CSF 的水平与脱离程度呈正相关，而 IL-1ra 和 CXCL10 与脱离时间有关。CXCL10 和 CCL11 的浓度与术前闪光值之间也存在正相关。进一步分析显示，B 级或 C 级增殖性玻璃体视网膜病变（PVR）眼的闪光值以及 CXCL10 和 CCL11 水平均显著升高。
我们的结果证实，RD 会引起明显的炎症反应，并伴有复杂的细胞因子网络。我们确定了与几种临床特征特别相关的蛋白质，这些特征可能导致光感受器变性和与 PVR 相关的复发性脱离。这些蛋白质可能代表改善 RD 手术解剖和功能结果的潜在治疗靶点。
