Department of Electrical Engineering and Information Systems, Graduate School of Engineering, The University of Tokyo, Tokyo 113-8656, Japan.
Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Tokyo 162-8666, Japan.
Proc Natl Acad Sci U S A. 2021 Sep 28;118(39). doi: 10.1073/pnas.2022300118.
Electrode arrays are widely used for multipoint recording of electrophysiological activities, and organic electronics have been utilized to achieve both high performance and biocompatibility. However, extracellular electrode arrays record the field potential instead of the membrane potential itself, resulting in the loss of information and signal amplitude. Although much effort has been dedicated to developing intracellular access methods, their three-dimensional structures and advanced protocols prohibited implementation with organic electronics. Here, we show an organic electrochemical transistor (OECT) matrix for the intracellular action potential recording. The driving voltage of sensor matrix simultaneously causes electroporation so that intracellular action potentials are recorded with simple equipment. The amplitude of the recorded peaks was larger than that of an extracellular field potential recording, and it was further enhanced by tuning the driving voltage and geometry of OECTs. The capability of miniaturization and multiplexed recording was demonstrated through a 4 × 4 action potential mapping using a matrix of 5- × 5-μm OECTs. Those features are realized using a mild fabrication process and a simple circuit without limiting the potential applications of functional organic electronics.
电极阵列被广泛用于多点记录电生理活动，而有机电子学已被用于实现高性能和生物相容性。然而，细胞外电极阵列记录的是场电位而不是膜电位本身，导致信息和信号幅度的损失。尽管已经做了很多努力来开发细胞内接入方法，但它们的三维结构和先进的方案阻止了它们与有机电子学的结合。在这里，我们展示了一种用于细胞内动作电位记录的有机电化学晶体管 (OECT) 矩阵。传感器矩阵的驱动电压同时引起电穿孔，从而可以用简单的设备记录细胞内动作电位。记录的峰值幅度大于细胞外场电位记录的幅度，并且通过调整 OECT 的驱动电压和几何形状进一步增强了幅度。通过使用 5 µm×5 µm 的 OECT 矩阵的 4×4 动作电位映射，展示了小型化和多路复用记录的能力。这些特性是通过使用温和的制造工艺和简单的电路实现的，而不会限制功能有机电子学的潜在应用。
