School of Biological Science and Technology, Liupanshui Normal University, Liupanshui Guizhou, China.
Human Resources Office, Liupanshui Normal University, Liupanshui Guizhou, China.
Bioengineered. 2021 Dec;12(1):6981-6995. doi: 10.1080/21655979.2021.1976043.
The ecological restoration of coal gangue can be achieved by planting (pigeon pea) because of its developed root system. The close relationships soil microorganisms have with plants are crucial for improving soil composition; the soil composition affects nutrient absorption. The microbial composition and function of soil planted with in reclaimed land were compared with soil that was not planted with (the control). Results showed that the dominant microflora in the soil significantly changed after planting . Before planting, the dominant microflora included members of the phyla Sulfobacteria and Acidobacteria. After planting, the dominant microflora contained bacteria from phyla and classes that included , and . Additionally, there were significant differences in the bacterial composition of each layer in soils planted with . Principal component analysis revealed that the interpretation degrees of the results for PC2 and PC3 axes were 10.46% and 3.87%, respectively. The dominant microflora were Vicinamibacterales, , and in the surface soil; and in the deep soil; and and in the mixed-layer soil. Function prediction analysis using the bioinformatics software package PICRUSt revealed that the abundance of operational taxonomic units corresponding to sigma 54-specific transcriptional regulators, serine threonine protein kinase, and histidine kinase increased by 111.2%, 56.8%, and 47.4%, respectively, after planting . This study provides a reference for interactions among microorganisms in reclaimed soils for guiding the development and restoration of waste coal gangue hills.
种植（木豆）可以实现煤矸石的生态恢复，因为它有发达的根系。土壤微生物与植物的密切关系对于改善土壤组成至关重要；土壤组成影响养分吸收。对在复垦土地上种植木豆和未种植木豆（对照）的土壤微生物组成和功能进行了比较。结果表明，种植后土壤中优势微生物群落发生了显著变化。种植前，优势微生物群落包括硫杆菌门和酸杆菌门的成员。种植后，优势微生物群落包含来自和的细菌门和纲，和。此外，种植木豆的土壤各层的细菌组成存在显著差异。主成分分析表明，PC2 和 PC3 轴的结果解释度分别为 10.46%和 3.87%。优势微生物群落在表土中为Vicinamibacterales、和；在深土中为和；在混合层土壤中为和。使用生物信息学软件包 PICRUSt 进行功能预测分析表明，种植后，与 sigma 54 特异性转录调节剂、丝氨酸苏氨酸蛋白激酶和组氨酸激酶对应的操作分类单位的丰度分别增加了 111.2%、56.8%和 47.4%。本研究为指导废煤矸石山的开发和恢复提供了复垦土壤中微生物相互作用的参考。
