Barua Chandana Choudhury, Ara Begum Shameem, Talukdar Archana, Datta Roy Jayanti, Buragohain Bhaben, Chandra Pathak Debesh, Kumar Sarma Dilip, Saikia Bora Rumi, Gupta Asheesh
Department of Veterinary Pharmacology & Toxicology, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati 781022, India.
ISRN Pharmacol. 2012;2012:948792. doi: 10.5402/2012/948792. Epub 2012 Aug 9.
Alternanthera brasiliana (L.) Kuntze (Amaranthaceae) is a herbaceous plant used against inflammation, cough, and diarrhea in Brazilian popular medicine. In our preliminary study, promising wound healing activity of methanol extract of leaves of A. brasiliana (MEAB) was observed in normal excision and incision wound models. Therefore, the present study was designed to investigate the wound healing activity along with the antioxidant enzyme profile during cutaneous excision immunocompromised wound after topical application of 5% w/w ointment of MEAB in rats. Immunocompromised state was induced by pretreatment with hydrocortisone (HC) at 40 mg/kg body weight (i.m.) in male rats. Following one-week pretreatment with HC, wounds were created. The vehicle, 5% (w/w) ointment of MEAB, or standard drug (Himax) was applied topically twice daily. Healing potential was evaluated by the rate of wound contraction, estimation of enzymatic and nonenzymatic antioxidants like catalase, SOD, GSH, protein, vitamin C, and hydroxyproline content, which was supported by histopathological study on the 8th day following wounding. There was significant increase in the enzymatic and nonenzymatic antioxidant parameters in the extract-reated group as compared to control group. Histopathological study revealed collagen deposition, fibroblast proliferation, angiogenesis, and development of basement membrane in A. brasiliana group. The results of the present investigation revealed significant wound healing activity of MEAB.
巴西Alternanthera brasiliana（L.）Kuntze（苋科）是一种草本植物，在巴西民间医学中用于治疗炎症、咳嗽和腹泻。在我们的初步研究中，在正常切除和切口伤口模型中观察到巴西Alternanthera brasiliana叶甲醇提取物（MEAB）具有良好的伤口愈合活性。因此，本研究旨在探讨在大鼠皮肤切除免疫受损伤口局部应用5%w/w MEAB软膏后，其伤口愈合活性以及抗氧化酶谱。通过给雄性大鼠腹腔注射40mg/kg体重的氢化可的松（HC）进行预处理来诱导免疫受损状态。用HC预处理一周后，制造伤口。每天局部涂抹两次赋形剂、5%（w/w）MEAB软膏或标准药物（希美克）。通过伤口收缩率、酶促和非酶促抗氧化剂（如过氧化氢酶、超氧化物歧化酶、谷胱甘肽、蛋白质、维生素C和羟脯氨酸含量）的测定来评估愈合潜力，这在受伤后第8天的组织病理学研究中得到了支持。与对照组相比，提取物处理组的酶促和非酶促抗氧化参数显著增加。组织病理学研究显示，巴西Alternanthera brasiliana组有胶原蛋白沉积、成纤维细胞增殖、血管生成和基底膜形成。本研究结果显示MEAB具有显著的伤口愈合活性。
