Xu Da-Ming, Li Qian, Yi Jing-Xing, Cai Xin-Jian, Xie Long, Fang Wei, Qiu Jin-Feng, Xu Cheng-Wei, He Chun-Ling, Xu Xian-Ru, Xu Jie-Song, Yin Jun
Division of Urological Surgery, Second Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong, People's Republic of China.
Division of Hematology, Second Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong, People's Republic of China.
Int J Gen Med. 2021 Sep 14;14:5573-5579. doi: 10.2147/IJGM.S326628. eCollection 2021.
In order to evaluate the effect of dyslipidemia on cellular or humoral immunity in patients, changes in the absolute number of lymphocyte subsets were detected.
Flow cytometry was applied to determine the absolute value of lymphocyte subsets: B cell, NK cell, CD4 T cell including the functional subset (CD4CD28), native subset (CD4CD45RACD62L), memory T cell subset (CD4CD45RA), CD8 T cell including the functional subset (CD8CD28) and activated subsets (CD8CD38 and CD8DR). The relationship between lymphocyte subsets and hypercholesterolemia and hypertriglyceridemia was analyzed.
The absolute values of CD19 B cell, CD3 T cell, CD4 Th cell, CD4CD28 cell, naive CD4 T cell and memory CD4 T cell in patients with dyslipidemia were markedly higher than those in healthy controls (<0.05). There was no significant difference between healthy controls and dyslipidemia patients in other lymphocyte subsets (>0.05). The absolute values of CD3 T cell and naive CD4 T cell were significantly positively correlated with hypercholesterolemia in peripheral blood (r=0.291 and 0.306, respectively, all <0.05). There was no significant correlation between hypertriglyceridemia and lymphocyte subsets (>0.05).
Dyslipidemia has potential effects on immune profiles in lymphocytes subsets, and changes in lymphocyte subsets in dyslipidemia patients may lead to immune dysfunction.
为了评估血脂异常对患者细胞免疫或体液免疫的影响，检测淋巴细胞亚群绝对数的变化。
应用流式细胞术测定淋巴细胞亚群的绝对值：B细胞、NK细胞、CD4 T细胞，包括功能亚群（CD4CD28）、天然亚群（CD4CD45RACD62L）、记忆T细胞亚群（CD4CD45RA）、CD8 T细胞，包括功能亚群（CD8CD28）和活化亚群（CD8CD38和CD8DR）。分析淋巴细胞亚群与高胆固醇血症和高甘油三酯血症之间的关系。
血脂异常患者的CD19 B细胞、CD3 T细胞、CD4 Th细胞、CD4CD28细胞、天然CD4 T细胞和记忆CD4 T细胞的绝对值显著高于健康对照组（<0.05）。其他淋巴细胞亚群在健康对照组和血脂异常患者之间无显著差异（>0.05）。外周血中CD3 T细胞和天然CD4 T细胞的绝对值与高胆固醇血症显著正相关（r分别为0.291和0.306，均<0.05）。高甘油三酯血症与淋巴细胞亚群之间无显著相关性（>0.05）。
血脂异常对淋巴细胞亚群的免疫谱有潜在影响，血脂异常患者淋巴细胞亚群的变化可能导致免疫功能障碍。
