Szabó Rubina Tunde, Kovács-Weber Maria, Erdélyi Márta, Balogh Krisztián, Fazekas Natasa, Horváth Ákos, Mézes Miklós, Kovács Balázs
Institute of Animal Husbandry, Faculty of Agricultural and Environmental Sciences, Szent István University, Páter Károly str. 1, H-2100, Gödöllo, Hungary.
Department of Nutrition, Faculty of Agricultural and Environmental Sciences, Szent István University, Páter Károly str. 1, H-2100, Gödöllo, Hungary.
Biol Futur. 2019 Dec;70(4):330-335. doi: 10.1556/019.70.2019.37. Epub 2019 Dec 1.
The aim of this study was to verify that the comet assay can be used to investigate the DNA damaging effects of T-2 and HT-2 toxins in the liver of broiler chickens. The comet assay is a favorable genotoxic analysis because it is cheap, simple, and can be used in many organisms and different tissues.
Male broiler chickens were fed with T-2/HT-2 toxins-contaminated diet for 14 days. The comet assay was successfully adapted to chicken liver cells, and the DNA damage was determined by a decrease in the comet parameter (DNA % in the tail) in the experimental groups.
The method of evaluation was found to be critical because DNA damage could not be detected exactly using the CometScore software, due to inaccurate separation of head and comet. However, this problem can be solved by visual evaluation.
In the case of the visual evaluation, each toxin-treated group differed significantly from the control group, indicating that the assay can be useful for the assessment of primary DNA damage caused by T-2/HT-2 toxins.
本研究的目的是验证彗星试验可用于研究T-2毒素和HT-2毒素对肉鸡肝脏的DNA损伤作用。彗星试验是一种有利的遗传毒性分析方法，因为它成本低廉、操作简单，可用于多种生物体和不同组织。
雄性肉鸡饲喂含T-2/HT-2毒素的污染饲料14天。彗星试验成功应用于鸡肝细胞，通过实验组彗星参数（尾部DNA百分比）的降低来确定DNA损伤。
发现评估方法至关重要，因为使用CometScore软件无法准确检测到DNA损伤，原因是头部和彗星部分分离不准确。然而，这个问题可以通过视觉评估来解决。
在视觉评估的情况下，每个毒素处理组与对照组有显著差异，表明该试验可用于评估T-2/HT-2毒素引起的原发性DNA损伤。
