影响鞭毛组装和功能的基因座定位于莱茵衣藻中一个不同寻常的连锁群。
Loci affecting flagellar assembly and function map to an unusual linkage group in Chlamydomonas reinhardtii.
作者信息
Ramanis Z, Luck D J
出版信息
Proc Natl Acad Sci U S A. 1986 Jan;83(2):423-6. doi: 10.1073/pnas.83.2.423.
Abstract
The uni1 mutant of Chlamydomonas reinhardtii lacks one of the paired flagella seen in wild-type cells. The missing flagellum is cis to the eye spot and at this site the basal body is incomplete, lacking a transition zone. Together with nine other loci affecting flagellar assembly and two loci affecting flagellar function uni1 defines an approximately equal to 100-centimorgan linkage group with a circular map. Measurements of gene-centromere distances on the uni linkage group are consistent with the ordering of loci determined by recombination analysis and place the centromere near pf29, a motility-defective mutant. In well-synchronized meiotic cycles, recombination frequencies between loci on the uni linkage group but not loci on other linkage groups show striking temperature sensitivity during a 1- to 2-hr period 5 days before meiosis.
摘要
莱茵衣藻的uni1突变体缺少野生型细胞中可见的一对鞭毛中的一根。缺失的鞭毛位于眼点的顺式位置，在此位置基体不完整，缺少过渡区。与其他九个影响鞭毛组装的基因座和两个影响鞭毛功能的基因座一起，uni1定义了一个约100厘摩的连锁群，其图谱呈圆形。对uni连锁群上基因与着丝粒距离的测量结果与通过重组分析确定的基因座顺序一致，并将着丝粒定位在pf29附近，pf29是一个运动缺陷型突变体。在同步良好的减数分裂周期中，uni连锁群上基因座之间的重组频率，而非其他连锁群上基因座之间的重组频率，在减数分裂前5天的1至2小时内表现出显著的温度敏感性。
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