Nagel J E, Han K, Coon P J, Adler W H, Bender B S
J Leukoc Biol. 1986 Apr;39(4):399-407. doi: 10.1002/jlb.39.4.399.
Elderly persons have increased morbidity and mortality due to bacterial infections. Since the polymorphonuclear leukocyte (PMN) is a major defense against bacterial infection, we utilized fluorescent microspheres and flow cytometry to examine phagocytosis by PMNs from 55 young and middle-aged adults (mean age 41.5 yrs) and two groups of elderly subjects: one group of 35 healthy individuals (mean age 74.1 years) living at home, and a second group of 11 residents (mean age 83.1 years) with severe mental and physical disabilities, living in a domiciliary care facility. We determined the percent phagocytic PMNs, the number of microspheres per PMN, and the number of microspheres per 100 PMNs. The mean number of microspheres per phagocytic PMN was similar for all groups. Statistically significant differences were found between the young and middle-aged group and the healthy or ill elderly groups for the percent phagocytic PMNs (75.3% vs 51.5% and 43.8%), and the number of microspheres per 100 phagocytic PMNs (197.3 vs 131.4 and 103.2). There were no significant differences in these parameters between healthy and debilitated elderly subjects. These data document that there is an age-related increase in representation of a population of PMNs which have a defect in phagocytic ability.
老年人因细菌感染导致发病率和死亡率增加。由于多形核白细胞（PMN）是抵御细菌感染的主要防线，我们利用荧光微球和流式细胞术检测了55名中青年成年人（平均年龄41.5岁）以及两组老年受试者的PMN吞噬作用：一组是35名居家健康个体（平均年龄74.1岁），另一组是11名居住在居家护理机构、患有严重精神和身体残疾的居民（平均年龄83.1岁）。我们测定了吞噬性PMN的百分比、每个PMN吞噬的微球数量以及每100个PMN吞噬的微球数量。所有组中每个吞噬性PMN吞噬的微球平均数量相似。在吞噬性PMN的百分比（75.3%对51.5%和43.8%）以及每100个吞噬性PMN吞噬的微球数量（197.3对131.4和103.2）方面，中青年组与健康或患病老年组之间存在统计学上的显著差异。健康和体弱的老年受试者在这些参数上没有显著差异。这些数据表明，吞噬能力存在缺陷的PMN群体在人群中的占比存在与年龄相关的增加。
