Scholefield Melissa, Church Stephanie J, Xu Jingshu, Patassini Stefano, Hooper Nigel M, Unwin Richard D, Cooper Garth J S
Centre for Advanced Discovery & Experimental Therapeutics, Division of Cardiovascular Sciences, School of Medical Sciences, Faculty of Biology, Medicine and Health, The University of Manchester, Manchester M13 9NT, UK.
School of Biological Sciences, Faculty of Science, University of Auckland, Private Bag 92 019, Auckland 1142, New Zealand.
Metabolites. 2021 Aug 25;11(9):569. doi: 10.3390/metabo11090569.
Pantothenic acid (vitamin B5) is an essential trace nutrient required for the synthesis of coenzyme A (CoA). It has previously been shown that pantothenic acid is significantly decreased in multiple brain regions in both Alzheimer's disease (ADD) and Huntington's disease (HD). The current investigation aimed to determine whether similar changes are also present in cases of Parkinson's disease dementia (PDD), another age-related neurodegenerative condition, and whether such perturbations might occur in similar regions in these apparently different diseases. Brain tissue was obtained from nine confirmed cases of PDD and nine controls with a post-mortem delay of 26 h or less. Tissues were acquired from nine regions that show high, moderate, or low levels of neurodegeneration in PDD: the cerebellum, motor cortex, primary visual cortex, hippocampus, substantia nigra, middle temporal gyrus, medulla oblongata, cingulate gyrus, and pons. A targeted ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) approach was used to quantify pantothenic acid in these tissues. Pantothenic acid was significantly decreased in the cerebellum ( = 0.008), substantia nigra ( = 0.02), and medulla ( = 0.008) of PDD cases. These findings mirror the significant decreases in the cerebellum of both ADD and HD cases, as well as the substantia nigra, putamen, middle frontal gyrus, and entorhinal cortex of HD cases, and motor cortex, primary visual cortex, hippocampus, middle temporal gyrus, cingulate gyrus, and entorhinal cortex of ADD cases. Taken together, these observations indicate a common but regionally selective disruption of pantothenic acid levels across PDD, ADD, and HD.
泛酸（维生素B5）是合成辅酶A（CoA）所需的一种必需微量营养素。先前的研究表明，在阿尔茨海默病（ADD）和亨廷顿病（HD）的多个脑区中，泛酸水平显著降低。当前的研究旨在确定在另一种与年龄相关的神经退行性疾病——帕金森病痴呆（PDD）病例中是否也存在类似变化，以及在这些明显不同的疾病中，这种扰动是否可能发生在相似区域。从9例确诊的PDD病例和9例死后延迟26小时或更短时间的对照者获取脑组织。组织取自PDD中显示高、中或低神经退行性变水平的9个区域：小脑、运动皮层、初级视觉皮层、海马体、黑质、颞中回、延髓、扣带回和脑桥。采用靶向超高效液相色谱 - 串联质谱（UHPLC-MS/MS）方法对这些组织中的泛酸进行定量。PDD病例的小脑（P = 0.008）、黑质（P = 0.02）和延髓（P = 本文中未提及延髓对应的P值，可能原文有误）中泛酸显著降低。这些发现反映了ADD和HD病例小脑以及HD病例黑质、壳核、额中回和内嗅皮质，ADD病例运动皮层、初级视觉皮层、海马体、颞中回、扣带回和内嗅皮质中泛酸的显著降低。综上所述，这些观察结果表明，PDD、ADD和HD中泛酸水平存在共同但具有区域选择性的破坏。 （注：原文中延髓部分P值缺失，译文按原文翻译并标注可能有误情况）
