Huang Xinglei, Zhang Yiyan, Wang Leilei, Dong Xinyi, Hu Weixin, Jiang Min, Chen Gang, An Gynheung, Xiong Fei, Wu Yunfei
Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops/Joint International Research Laboratory of Agriculture and Agri-Product Safety, Yangzhou University, Yangzhou, China.
Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, China.
Front Plant Sci. 2021 Sep 10;12:703034. doi: 10.3389/fpls.2021.703034. eCollection 2021.
Carbon and nitrogen antagonistically regulate multiple developmental processes. However, the molecular mechanism affecting nitrogen metabolism by sucrose transport remains poorly defined. Previously, we noted that () mediated sucrose transport by binding to the promoter regions of (), , and . Here, we note that promotes nitrogen uptake and then maintains the ratio of fresh weight to dry weight in seedling plants and the effective leaf blade at flowering stages. Mutants of the sucrose transporter gene displayed a phenotype similar to that of . By microarray analysis and qRT-PCR in mutant plants, affected the transcription level of amino acid metabolism-related genes. We further found that mainly amino acid contents were reduced in flag leaves but increased in seeds. Both sugar and organic nitrogen changes caused the ratio of fresh weight to dry weight to decrease in mutant seedling plants and mature leaves, which might result in vigorous reduced metabolic activity and become less susceptible to stress. These results demonstrated that affected nitrogen metabolism by sugar distribution in rice, which provided new insight that coordinated with C and N balance to maintain plant growth activity.
碳和氮对多种发育过程具有拮抗调节作用。然而，蔗糖转运影响氮代谢的分子机制仍不清楚。此前，我们发现（）通过与（）、（）和（）的启动子区域结合来介导蔗糖转运。在此，我们发现（）促进氮吸收，进而维持幼苗期植株鲜重与干重的比例以及开花期有效叶片的该比例。蔗糖转运蛋白基因（）的突变体表现出与（）类似的表型。通过对（）突变体植株进行微阵列分析和定量逆转录聚合酶链反应，（）影响了氨基酸代谢相关基因的转录水平。我们进一步发现，旗叶中主要氨基酸含量降低，而种子中则升高。糖和有机氮的变化均导致（）突变体幼苗植株和成熟叶片的鲜重与干重比值降低，这可能导致代谢活性显著降低且对胁迫的敏感性降低。这些结果表明，（）通过水稻中的糖分配影响氮代谢，这为（）与碳氮平衡协同维持植物生长活性提供了新的见解。
