Department of Respiratory and Critical Care, Beijing Tian Tan Hospital, Capital Medical University, Beijing, China.
Front Cell Infect Microbiol. 2021 Sep 9;11:694756. doi: 10.3389/fcimb.2021.694756. eCollection 2021.
Lower respiratory tract infections are associated with high morbidity and mortality and significant clinical harm. Due to the limited ability of traditional pathogen detection methods, anti-infective therapy is mostly empirical. Therefore, it is difficult to adopt targeted drug therapy. In recent years, metagenomic next-generation sequencing (mNGS) technology has provided a promising means for pathogen-specific diagnosis and updated the diagnostic strategy for lower respiratory tract infections. This article reviews the diagnostic value of mNGS for lower respiratory tract infections, the impact of different sampling methods on the detection efficiency of mNGS, and current technical difficulties in the clinical application of mNGS.
下呼吸道感染与高发病率和死亡率以及显著的临床危害有关。由于传统病原体检测方法的能力有限，抗感染治疗大多是经验性的。因此，很难采用靶向药物治疗。近年来，宏基因组下一代测序（mNGS）技术为病原体特异性诊断提供了一种很有前途的手段，并更新了下呼吸道感染的诊断策略。本文综述了 mNGS 在下呼吸道感染中的诊断价值、不同采样方法对 mNGS 检测效率的影响，以及 mNGS 在临床应用中的当前技术难点。
