National Engineering Laboratory for Cereal Fermentation Technology (NELCF), School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China.
Jiangsu Provincial Research Center for Bioactive Product Processing Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China.
Genes (Basel). 2021 Aug 25;12(9):1311. doi: 10.3390/genes12091311.
The calcium/calcineurin signalling pathway is required for cell survival under various environmental stresses. Using , we explored the mechanism underlying calcium-regulated homeostasis of intracellular reactive oxygen species (ROS). We found that deletion of acyltransferase Akr1 and C-5 sterol desaturase Erg3 increased the intracellular ROS levels and cell death, and this could be inhibited by the addition of calcium. The hexose transporter Hxt1 and the amino acid permease Agp1 play crucial roles in maintaining intracellular ROS levels, and calcium induced the expression of the and genes. The cytosolic calcium concentration was decreased in both the and mutants relative to wild-type cells, potentially lowering basal expression of and . Moreover, the calcium/calcineurin signalling pathway also induced the expression of and , indicating that Akr1 and Erg3 might perform functions that help yeast cells to survive under high calcium concentrations. Our results provided mechanistic insight into how calcium regulated intracellular ROS levels in yeast.
钙调神经磷酸酶信号通路是细胞在各种环境胁迫下存活所必需的。利用 ，我们探讨了钙调节细胞内活性氧（ROS）稳态的机制。我们发现酰基转移酶 Akr1 和 C-5 甾醇去饱和酶 Erg3 的缺失会增加细胞内 ROS 水平和细胞死亡，而这可以通过添加钙来抑制。己糖转运蛋白 Hxt1 和氨基酸渗透酶 Agp1 在维持细胞内 ROS 水平方面发挥着关键作用，钙诱导 和 基因的表达。与野生型细胞相比，细胞质钙离子浓度在 和 突变体中降低，可能降低了 和 的基础表达。此外，钙调神经磷酸酶信号通路还诱导 和 的表达，表明 Akr1 和 Erg3 可能具有帮助酵母细胞在高钙浓度下存活的功能。我们的研究结果为钙如何调节酵母细胞内 ROS 水平提供了机制上的见解。
