Storch Christiane, Fuhrmann Herbert, Schoeniger Axel
Institute of Biochemistry, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 1, 04103 Leipzig, Germany.
Animals (Basel). 2021 Aug 30;11(9):2542. doi: 10.3390/ani11092542.
Osteoarthritis the quality and span of life in horses. Previous studies focused on nasal cartilage as a possible source for autologous chondrocyte implantation (ACI) in cartilage defects in humans. "HOX gene-negative" nasal chondrocytes adapted articular HOX patterns after implantation into caprine joint defects and produced cartilage matrix proteins. We compared the HOX gene profile of equine chondrocytes of nasal septum, anterior and posterior fetlock to identify nasal cartilage as a potential source for ACI in horses. Cartilage was harvested from seven horses after death and derived chondrocytes were cultured in a monolayer to fourth subcultivation. HOX A3, D1, D8 and chondrocyte markers COL2 and SOX9 were analyzed with qPCR in chondrocytes of three different locations obtained during passage 0 and passage 2. HOX gene expression showed no significant differences between the locations but varied significantly between the horses. HOX genes and SOX9 remained stable during culturing. Cultured nasal chondrocytes may be a target for future research in cell-based regenerative therapies in equine osteoarthritis. The involvement of HOX genes in the high regenerative and adaptive potential of nasal chondrocytes observed in previous studies could not be confirmed.
骨关节炎对马的生活质量和寿命的影响。先前的研究聚焦于鼻软骨，将其作为人类软骨缺损中自体软骨细胞植入（ACI）的可能来源。“HOX基因阴性”的鼻软骨细胞在植入山羊关节缺损后适应了关节HOX模式并产生软骨基质蛋白我们比较了鼻中隔软骨细胞、前后跗关节软骨细胞HOX基因谱，以确定鼻软骨作为马ACI潜在供体的可能性。在7匹马死后采集软骨，将所得软骨细胞进行单层培养至第4代传代培养。在第0代和第2代传代培养时获得的三个不同位置的软骨细胞用qPCR分析HOX A基因、D1、D8以及软骨细胞标记物COL2和SOX基因表达在不同位置之间无显著差异，但不同马匹之间差异显著HOX基因表达和SOX基因在培养过程中保持稳定。培养的鼻软骨细胞可能是马骨关节炎基于细胞的再生疗法未来研究的一个靶点。先前研究中观察到的HOX基因在鼻软骨细胞高再生和适应潜力中的作用无法得到证实。
