National Engineering Research Center for Floriculture, Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, Beijing Laboratory of Urban and Rural Ecological Environment, School of Landscape Architecture, Beijing Forestry University, Beijing 100083, China.
College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, China.
Int J Mol Sci. 2021 Nov 5;22(21):12010. doi: 10.3390/ijms222112010.
() genes are members of the well-known MADS-box gene family that play a key role in regulating vital developmental processes in plants. are perennial herbs that exhibit continuous flowering development and have been extensively used in landscaping. However, there are few reports on the regulatory mechanism of flowering in . To better understand the molecular basis of floral formation of , we identified and characterized the -like gene from the cultivar 'Kanai Sensei'. Quantitative RT-PCR (qRT-PCR) indicated that transcript was mainly expressed in the vegetative growth stage and had the highest expression in leaves, low expression in petals, pedicels and fruits, and no expression in pistils. The HkSVP encoded protein was localized in the nucleus of protoplasts and the nucleus of onion epidermal cells. Yeast two hybrid assay revealed that HKSVP interacted with AP1 and TFL1. Moreover, overexpression of in resulted in delayed flowering and abnormal phenotypes, including enriched trichomes, increased basal inflorescence branches and inhibition of inflorescence formation. These observations suggest that the gene may play an important role in maintaining vegetative growth by participating in the construction of inflorescence structure and the development of flower organs.
() 基因是众所周知的 MADS-box 基因家族的成员，在植物的重要发育过程中起着关键作用。() 是多年生草本植物，表现出连续的开花发育，被广泛用于景观美化。然而，关于 () 的开花调控机制的报道很少。为了更好地理解 () 花形成的分子基础，我们从 () 品种“Kanai Sensei”中鉴定并表征了类基因 ()。定量 RT-PCR(qRT-PCR) 表明，() 转录本主要在营养生长阶段表达，在叶片中表达最高，在花瓣、花梗和果实中表达较低，在雌蕊中无表达。HkSVP 编码的蛋白定位于原生质体和洋葱表皮细胞的细胞核中。酵母双杂交实验表明，HKSVP 与 AP1 和 TFL1 相互作用。此外，在 () 中过表达 () 导致开花延迟和异常表型，包括丰富的毛状体、增加的基部花序分枝和花序形成的抑制。这些观察结果表明，该基因可能通过参与花序结构的构建和花器官的发育，在维持营养生长中发挥重要作用。
