Kim Jooseong, Kim Sukyoung, Song Inhwan
Department of Biomedical Engineering, Yeungnam University, 170 Hyeonchung-ro, Nam-gu, Daegu 42415, Korea.
School of Materials Science and Engineering, Yeungnam University, 280 Daehak-ro, Gyeongsan 38541, Korea.
Materials (Basel). 2021 Sep 14;14(18):5300. doi: 10.3390/ma14185300.
Octacalcium phosphate (OCP) is a precursor of biological apatite crystals that has attracted attention as a possible bone substitute. On the other hand, few studies have examined this material at the experimental level due to the limitations on OCP mass production. Recently, mass production technology of OCP was developed, and the launch of OCP bone substitutes is occurring. In this study, the bone regeneration capacity of OCP products was compared with two of the most clinically used materials: heat-treated bovine bone (BHA) and sintered biphasic calcium phosphate (BCP). Twelve rabbits were used, and defects in each tibia were filled with OCP, BHA, BCP, and left unfilled as control (CON). The tibias were harvested at 4 and 12 weeks, and 15 μm slides were prepared using the diamond grinding method after being embedded in resin. Histological and histomorphometric analyses were performed to evaluate the bone regeneration ability and mechanism. The OCP showed significantly higher resorption and new bone formation in both periods analysed ( < 0.05). Overall, OCP bone substitutes can enhance bone regeneration significantly by activating osteoblasts and a rapid phase transition of OCP crystals to biological apatite crystals (mineralization), as well as providing additional space for new bone formation by rapid resorption.
八钙磷酸酯（OCP）是生物磷灰石晶体的前体，作为一种可能的骨替代物受到了关注。另一方面，由于OCP大规模生产的限制，很少有研究在实验层面上对这种材料进行研究。最近，OCP的大规模生产技术得到了发展，并且OCP骨替代物正在上市。在本研究中，将OCP产品的骨再生能力与两种临床上最常用的材料进行了比较：热处理牛骨（BHA）和烧结双相磷酸钙（BCP）。使用了12只兔子，每个胫骨的缺损分别用OCP、BHA、BCP填充，未填充作为对照（CON）。在4周和12周时收获胫骨，在树脂包埋后使用金刚石研磨法制备15μm的切片。进行组织学和组织形态计量学分析以评估骨再生能力和机制。在两个分析时期，OCP均显示出显著更高的吸收和新骨形成（<0.05）。总体而言，OCP骨替代物可以通过激活成骨细胞以及OCP晶体快速向生物磷灰石晶体转变（矿化），以及通过快速吸收为新骨形成提供额外空间，从而显著增强骨再生。
