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用光镊技术对人类红细胞进行横向变形研究。

Lateral Deformation of Human Red Blood Cells by Optical Tweezers.

作者信息

Yale Pavel, Kouacou Michel A, Konin Jean-Michel E, Megnassan Eugène, Zoueu Jérémie T

机构信息

Laboratoire de Physique Fondamentale et Appliquée, UNA, Abidjan BP 801, Côte d'Ivoire.

Laboratoire d'Instrumentation, Image et Spectroscopie, INPHB, Yamoussoukro BP 1093, Côte d'Ivoire.

出版信息

Micromachines (Basel). 2021 Aug 27;12(9):1024. doi: 10.3390/mi12091024.

DOI:10.3390/mi12091024
PMID:34577667
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8468094/
Abstract

In this paper, we studied the lateral deformation of human red blood cells (RBCs) during lateral indentation by an optically trapped silica bead with a diameter of 4.5 µm (Bangs Laboratories, Inc. Fishers, IN, USA). The images were captured using a CCD camera and the Boltzmann statistics method was used for force calibration. Using the Hertz model, we calculated and compared the elastic stiffness resulting from the lateral force, showing that the differences are important and that the force should be considered. Besides the lateral component, this setup also allowed us to examine the lateral cell-bead interaction. The mean values of the cell shear stiffness measured during indentation were 3.37 ± 0.40 µN/m for biconcave RBCs, 3.48 ± 0.23 µN/m for spherical RBCs, and 3.80 ± 0.22 µN/m for crenelated RBCs, respectively. These results show that this approach can be used as a routine method for RBC study, because it enabled us to manipulate the cell without contact with the wall.

摘要

在本文中，我们研究了直径为4.5 µm的光学捕获二氧化硅微珠（美国印第安纳州费舍尔市邦斯实验室公司）对人红细胞（RBC）进行侧向压痕时的横向变形。使用电荷耦合器件（CCD）相机捕获图像，并采用玻尔兹曼统计方法进行力校准。利用赫兹模型，我们计算并比较了侧向力产生的弹性刚度，结果表明差异显著，应考虑该力。除了侧向分量外，该装置还使我们能够研究细胞与微珠之间的侧向相互作用。压痕过程中测得的双凹红细胞、球形红细胞和锯齿状红细胞的细胞剪切刚度平均值分别为3.37±0.40µN/m、3.48±0.23µN/m和3.80±0.22µN/m。这些结果表明，该方法可作为研究红细胞的常规方法，因为它使我们能够在不与壁接触的情况下操纵细胞。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ed9/8468094/96d6a9a6ad37/micromachines-12-01024-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ed9/8468094/0f2797e76110/micromachines-12-01024-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ed9/8468094/caa71ea594be/micromachines-12-01024-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ed9/8468094/472a5db7c025/micromachines-12-01024-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ed9/8468094/96d6a9a6ad37/micromachines-12-01024-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ed9/8468094/0f2797e76110/micromachines-12-01024-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ed9/8468094/caa71ea594be/micromachines-12-01024-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ed9/8468094/472a5db7c025/micromachines-12-01024-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ed9/8468094/96d6a9a6ad37/micromachines-12-01024-g004.jpg

相似文献

[1]
Lateral Deformation of Human Red Blood Cells by Optical Tweezers.

Micromachines (Basel). 2021-8-27

[2]
Deformation behaviour of stomatocyte, discocyte and echinocyte red blood cell morphologies during optical tweezers stretching.

Biomech Model Mechanobiol. 2020-10

[3]
Automatic real time evaluation of red blood cell elasticity by optical tweezers.

Rev Sci Instrum. 2015-5

[4]
Deformability and intrinsic material properties of neonatal red blood cells.

Blood. 1986-5

[5]
New Detector Sensitivity Calibration and the Calculation of the Interaction Force between Particles Using an Optical Tweezer.

Micromachines (Basel). 2018-8-24

[6]
Radiation pressure on a biconcave human Red Blood Cell and the resulting deformation in a pair of parallel optical traps.

J Biophotonics. 2014-10

[7]
Mechanical characterization of human red blood cells under different osmotic conditions by robotic manipulation with optical tweezers.

IEEE Trans Biomed Eng. 2010-2-18

[8]
Effect of the size and shape of a red blood cell on elastic light scattering properties at the single-cell level.

Biomed Opt Express. 2011-7-1

[9]
Investigation of red blood cell mechanical properties using AFM indentation and coarse-grained particle method.

Biomed Eng Online. 2017-12-19

[10]
Multifunctional manipulation of red blood cells using optical tweezers.

J Biophotonics. 2022-2

引用本文的文献

[1]
In vitro investigation of the mechanics of fixed red blood cells based on optical trap micromanipulation and image analysis.

Biomed Opt Express. 2024-5-16

[2]
Mechanical Characterization of the Erythrocyte Membrane Using a Capacitor-Based Technique.

Micromachines (Basel). 2024-4-28

[3]
Analysis of Polarization Images in the Microphysical Blood Parameters Research for the Hematocrit Diagnostics.

Micromachines (Basel). 2022-12-16

本文引用的文献

[1]
New Detector Sensitivity Calibration and the Calculation of the Interaction Force between Particles Using an Optical Tweezer.

Micromachines (Basel). 2018-8-24

[2]
Optical tweezers study of red blood cell aggregation and disaggregation in plasma and protein solutions.

J Biomed Opt. 2016-3

[3]
Calibration of optical tweezers for in vivo force measurements: how do different approaches compare?

Biophys J. 2014-9-16

[4]
Trapping red blood cells in living animals using optical tweezers.

Nat Commun. 2013

[5]
Cell visco-elasticity measured with AFM and optical trapping at sub-micrometer deformations.

PLoS One. 2012-9-19

[6]
Metabolic remodeling of the human red blood cell membrane.

Proc Natl Acad Sci U S A. 2010-1-6

[7]
Biomechanical forces promote embryonic haematopoiesis.

Nature. 2009-6-25

[8]
Cell and molecular mechanics of biological materials.

Nat Mater. 2003-11

[9]
The optical stretcher: a novel laser tool to micromanipulate cells.

Biophys J. 2001-8

[10]
A new determination of the shear modulus of the human erythrocyte membrane using optical tweezers.

Biophys J. 1999-2

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