Yale Pavel, Kouacou Michel A, Konin Jean-Michel E, Megnassan Eugène, Zoueu Jérémie T
Laboratoire de Physique Fondamentale et Appliquée, UNA, Abidjan BP 801, Côte d'Ivoire.
Laboratoire d'Instrumentation, Image et Spectroscopie, INPHB, Yamoussoukro BP 1093, Côte d'Ivoire.
Micromachines (Basel). 2021 Aug 27;12(9):1024. doi: 10.3390/mi12091024.
In this paper, we studied the lateral deformation of human red blood cells (RBCs) during lateral indentation by an optically trapped silica bead with a diameter of 4.5 µm (Bangs Laboratories, Inc. Fishers, IN, USA). The images were captured using a CCD camera and the Boltzmann statistics method was used for force calibration. Using the Hertz model, we calculated and compared the elastic stiffness resulting from the lateral force, showing that the differences are important and that the force should be considered. Besides the lateral component, this setup also allowed us to examine the lateral cell-bead interaction. The mean values of the cell shear stiffness measured during indentation were 3.37 ± 0.40 µN/m for biconcave RBCs, 3.48 ± 0.23 µN/m for spherical RBCs, and 3.80 ± 0.22 µN/m for crenelated RBCs, respectively. These results show that this approach can be used as a routine method for RBC study, because it enabled us to manipulate the cell without contact with the wall.
在本文中，我们研究了直径为4.5 µm的光学捕获二氧化硅微珠（美国印第安纳州费舍尔市邦斯实验室公司）对人红细胞（RBC）进行侧向压痕时的横向变形。使用电荷耦合器件（CCD）相机捕获图像，并采用玻尔兹曼统计方法进行力校准。利用赫兹模型，我们计算并比较了侧向力产生的弹性刚度，结果表明差异显著，应考虑该力。除了侧向分量外，该装置还使我们能够研究细胞与微珠之间的侧向相互作用。压痕过程中测得的双凹红细胞、球形红细胞和锯齿状红细胞的细胞剪切刚度平均值分别为3.37±0.40µN/m、3.48±0.23µN/m和3.80±0.22µN/m。这些结果表明，该方法可作为研究红细胞的常规方法，因为它使我们能够在不与壁接触的情况下操纵细胞。
