Jack I, Seshadri R, Garson M, Michael P, Callen D, Zola H, Morley A
Cancer Genet Cytogenet. 1986 Jan 15;19(3-4):261-9. doi: 10.1016/0165-4608(86)90055-5.
A cell line (RCH-ACV) was established from a bone marrow sample of a child with acute lymphoblastic leukemia (ALL). The cell line lacked Epstein-Barr virus nuclear antigen and exhibited a recently described nonrandom chromosome translocation, 1;19, thought to be associated with pre-B-ALL and poor prognosis. Banding studies confirm that the breakpoint of chromosome #19 occurs at p13.3. Cell surface marker analysis using a panel of monoclonal antibodies revealed markers consistent with common ALL phenotype. Although the cells did not show cytoplasmic immunoglobulin, studies of the immunoglobulin gene rearrangement confirmed the pre-B phenotype. This cell line could be of great value to studies of the role of the specific translocation 1;19 in the etiology of pre-B-ALL.
从一名急性淋巴细胞白血病（ALL）患儿的骨髓样本中建立了一个细胞系（RCH-ACV）。该细胞系缺乏EB病毒核抗原，并表现出一种最近描述的非随机染色体易位，即1；19，被认为与前B-ALL及不良预后相关。染色体显带研究证实19号染色体的断点位于p13.3。使用一组单克隆抗体进行的细胞表面标志物分析显示出与常见ALL表型一致的标志物。尽管这些细胞未显示胞质免疫球蛋白，但免疫球蛋白基因重排研究证实了前B表型。该细胞系对于研究特定的1；19易位在前B-ALL病因学中的作用可能具有重要价值。
