Van der Velden U, Van Winkelhoff A J, Abbas F, De Graaff J
J Clin Periodontol. 1986 Mar;13(3):243-8. doi: 10.1111/j.1600-051x.1986.tb01467.x.
The purpose of the present study was to investigate the significance of oral mucosal surfaces as a habitat of periodontopathic micro-organisms. For this investigation, 7 young adults were selected for an experimental gingivitis study. At the start of the experiment, bacterial samples were taken by means of a sterile swab from 4 oral mucosal surfaces: the buccal mucosa, the vestibular fold, the dorsum of the tongue and a tonsil. All individuals were asked to abstain from oral hygiene procedures in 1 quadrant of the mouth for a period of 23 days. At the end of this period, plaque samples were taken from 4 tooth surfaces by means of a sterile periodontal probe just coronal from the gingival margin. The study included evaluation of spirochetes and motile organisms by means of phase-contrast microscopy and of black-pigmented Bacteroides, Fusobacterium, Actinomyces, Actinobacillus actinomycetemcomitans and Capnocytophaga by means of cultural studies. Results showed a relationship between the prevalence of motile organisms and black-pigmented Bacteroides in samples from both the mucosal surfaces and the 23-day-old plaque. In addition, a relationship was found between the presence of motile organisms on the dorsum of the tongue at the start of the experiment and their prevalence in the 23-day-old plaque. It was suggested that the dorsum of the tongue may function as a nidus for periodontopathic micro-organisms.
本研究的目的是调查口腔黏膜表面作为牙周病微生物栖息地的意义。为了进行这项调查，选择了7名青年成年人进行实验性牙龈炎研究。在实验开始时，用无菌拭子从4个口腔黏膜表面采集细菌样本：颊黏膜、前庭皱襞、舌背和扁桃体。要求所有个体在口腔的1个象限内停止口腔卫生措施，为期23天。在这段时间结束时，用无菌牙周探针从牙龈边缘冠方的4个牙面采集菌斑样本。该研究包括通过相差显微镜评估螺旋体和运动性微生物，以及通过培养研究评估产黑色素类杆菌、梭杆菌、放线菌、伴放线放线杆菌和二氧化碳嗜纤维菌。结果显示，在黏膜表面和23日龄菌斑的样本中，运动性微生物和产黑色素类杆菌的流行率之间存在关联。此外，在实验开始时舌背上运动性微生物的存在与其在23日龄菌斑中的流行率之间也发现了关联。有人提出，舌背可能是牙周病微生物的滋生地。
