次氯酸含漱液对牙周病患者唾液细菌（包括金黄色葡萄球菌）的影响：一项随机对照试验。
Effects of hypochlorous acid mouthwash on salivary bacteria including Staphylococcus aureus in patients with periodontal disease: a randomized controlled trial.
机构信息
School of Dentistry, College of Dental Medicine, Kaohsiung Medical University, No.100, Shih-Chuan 1st Road, Kaohsiung, 80708, Taiwan.
Wenhsin Dental Clinic, 1F, No. 182, Zhongzheng 2nd Rd., Xinxing Dist, Kaohsiung, Taiwan.
出版信息
BMC Oral Health. 2023 Sep 28;23(1):698. doi: 10.1186/s12903-023-03358-4.
BACKGROUND
The effects of a low concentration of hypochlorous acid (HOCl) mouthwash on salivary bacteria remained unclear. We aimed to evaluate the antibacterial effects of 100 ppm HOCl mouthwash on salivary bacteria, including Staphylococcus aureus (S. aureus), in patients with periodontal disease (PD).
METHODS
Patients with PD were randomized into mouthwash-only (MW, n = 26) and mouthwash with periodontal flosser (MWPF, n = 27) groups. Patients without PD were selected for the control group (n = 30). S. aureus culture and saliva samples (before and after the intervention) were collected for bacterial DNA extraction. A real-time polymerase chain reaction assay and serial dilutions of S. aureus culture and saliva samples were used to measure the salivary bacteria total count (SBTC) and confirm the antibacterial effects of the mouthwash using S. aureus.
RESULTS
No significant difference in demographic data was observed among the three groups. Before the intervention, the baseline SBTC of the MW and MWPF groups was significantly higher than that of the control group. After the mouthwash rinses, the SBTC data significantly changed in the MW and MWPF groups only (by 62.4% and 77.4%, respectively). After the base-2 log-transformation of the SBTC data, a similar trend was observed. Linear regression revealed that baseline SBTC and the MWPF intervention significantly affected SBTC reduction percentage by volume. After incubation with 10% (v/v) of mouthwash, the survival rates of 10 and 10 colony-forming units/mL of S. aureus were 0.51% ± 0.06% and 1.42% ± 0.37%, respectively.
CONCLUSIONS
These study results indicated that 100 ppm HOCl mouthwash treatment could effectively reduce SBTC in patients with PD and the abundance of S. aureus. It provides that the HOCl mouthwash can be an option for individuals to help control SBTC, especially in patients with PD.
TRIAL REGISTRATION
The study protocol was approved by the Institutional Review Board of Kaohsiung Medical University Hospital (KMUHIRB-F(I)-20200042) on 20/03/2020 and retrospectively registered at ClinicalTrial.gov (NCT05372835) on 13/05/2022.
背景
低浓度次氯酸（HOCl）漱口水对唾液细菌的影响尚不清楚。我们旨在评估 100ppm HOCl 漱口水对牙周病（PD）患者唾液细菌（包括金黄色葡萄球菌[S. aureus]）的抗菌作用。
方法
将 PD 患者随机分为仅使用漱口水（MW，n=26）和使用漱口水加牙周牙线（MWPF，n=27）组。选择无 PD 的患者作为对照组（n=30）。采集唾液样本（干预前后）进行 S. aureus 培养和细菌 DNA 提取。采用实时聚合酶链反应检测和 S. aureus 培养物和唾液样本的连续稀释法，测量唾液细菌总数（SBTC），并确认漱口水的抗菌作用。
结果
三组间人口统计学数据无显著差异。干预前，MW 和 MWPF 组的基线 SBTC 明显高于对照组。漱口后，仅 MW 和 MWPF 组的 SBTC 数据发生显著变化（分别降低 62.4%和 77.4%）。对 SBTC 数据进行以 2 为底的对数转换后，观察到类似的趋势。线性回归显示，基线 SBTC 和 MWPF 干预显著影响 SBTC 减少百分比。孵育 10%（v/v）漱口水后，S. aureus 10 和 10 菌落形成单位/mL 的存活率分别为 0.51%±0.06%和 1.42%±0.37%。
结论
这些研究结果表明，100ppm HOCl 漱口水治疗可有效降低 PD 患者的 SBTC 和金黄色葡萄球菌丰度。这表明 HOCl 漱口水可以作为一种控制 SBTC 的选择，特别是在 PD 患者中。
试验注册
该研究方案于 2020 年 3 月 20 日获得高雄医学大学附设中和纪念医院机构审查委员会（KMUHIRB-F(I)-20200042）批准，并于 2022 年 5 月 13 日在 ClinicalTrials.gov 进行了回顾性注册（NCT05372835）。
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