Institute of Food Science & Technology, Fuzhou University, Fuzhou 350108, China.
Nutrients. 2020 Mar 31;12(4):966. doi: 10.3390/nu12040966.
Tea is the most widely consumed beverages next to water, however little is known about the influence of sustained tea consumption on the oral bacteria of healthy adults. In this study, three oral healthy adults were recruited and instructed to consume 1.0 L of oolong tea infusions (total polyphenol content, 2.83 g/L) daily, for eight weeks. Salivary microbiota pre-, peri-, and post-treatment were fully compared by high-throughput 16S rRNA sequencing and multivariate statistical analysis. It was revealed that oolong tea consumption reduced salivary bacterial diversity and the population of some oral disease related bacteria, such as sp., , , , and . Moreover, via correlation network and Venn diagram analyses, seven bacterial taxa, including sp. (OTU_1), sp. (OTU_33), sp. (OTU_696), spp. (OTU_133 and OTU_23), (OTU_42), and (OTU_6), were significantly altered after oolong tea consumption, and presented robust strong connections (|r| > 0.9 and < 0.05) with other oral microbiota. These results suggest sustained oolong tea consumption would modulate salivary microbiota and generate potential oral pathogen preventative benefits. Additionally, diverse responses to oolong tea consumption among subjects were also noticed.
茶是仅次于水的最广泛消费的饮料，然而，人们对持续饮茶对健康成年人口腔细菌的影响知之甚少。在这项研究中，招募了三名口腔健康成年人，并指示他们每天饮用 1.0 升乌龙茶提取物（总多酚含量为 2.83 克/升），持续八周。通过高通量 16S rRNA 测序和多变量统计分析，全面比较了治疗前、治疗中和治疗后的唾液微生物组。结果表明，乌龙茶的饮用降低了唾液细菌的多样性和一些与口腔疾病相关的细菌的种群，如 sp.、 sp.、 sp.、 sp.、 sp.。此外，通过相关网络和 Venn 图分析，七种细菌分类群，包括 sp.（OTU_1）、 sp.（OTU_33）、 sp.（OTU_696）、 spp.（OTU_133 和 OTU_23）、 （OTU_42）和 （OTU_6），在乌龙茶饮用后发生了显著变化，与其他口腔微生物群具有稳健的强关联（|r|>0.9 和 < 0.05）。这些结果表明，持续饮用乌龙茶会调节唾液微生物群，并产生潜在的预防口腔病原体的益处。此外，还注意到受试者对乌龙茶的饮用存在不同的反应。
